Short-term effects of extremely low-frequency pulsed electromagnetic field and pulsed low-level laser therapy on rabbit model of corneal alkali burn
Short-term effects of extremely low-frequency pulsed electromagnetic field and pulsed low-level laser therapy on rabbit model of corneal alkali burn
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DOI：
10.1016/j.exer.2016.01.007
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发表时间：
2016-04-01
影响因子：
3.4
通讯作者：
AfsarAski, Sasha
中科院分区：
文献类型：
--
作者：
Kanavi, Mozhgan Rezaei;Tabeie, Faraj;AfsarAski, Sasha
本研究探讨了极低频脉冲电磁场（ELF-PEMF）联合低强度激光（LLLT）治疗兔碱烧伤角膜的效果。将50只家兔50只碱烧伤角膜分为5组：2 mT强度（ELF 2）的ELF- pemf治疗，每天2 h；每天两次，每次30分钟；ELF- pemf和LLLT联合（ELF + LLLT）；医疗（MT）；对照组（即不进行治疗）。于第0、2、7、14天进行角膜的临床检查和数码摄影。对家兔实施安乐死后，采用组织病理学方法对染病眼进行观察。比较两组临床及组织病理学结果。在第7天和第14天，ELF、LLLT、ELF + LLLT和MT组的角膜缺损面积无明显差异。除对照组外，在常规组织病理学和CD31免疫组化中，没有一个研究组显示出明显的角膜新生血管。MT组的角质细胞损失明显高于ELF、LLLT和ELF + LLLT组。LLLT组中至重度间质炎症与MT组相当，显著低于其他组。综上所述，LLLT联合ELF治疗角膜碱烧伤的效果并不优于单纯使用ELF或单独使用LLLT。然而，考虑到LLLT的角膜炎症强度较低，角膜细胞损失率较低，这种治疗方法可能优于其他提出的治疗碱烧伤角膜的方法。(C) 2016 Elsevier Ltd.版权所有。
This study was conducted to investigate the effect of combining extremely low frequency pulsed electromagnetic field (ELF-PEMF) and low-level laser therapy (LLLT) on alkali-burned rabbit corneas. Fifty alkali-burned corneas of 50 rabbits were categorized into five groups: ELF-PEMF therapy with 2 mT intensity (ELF 2) for 2 h daily; LLLT for 30 min twice daily; combined ELF-PEMF and LLLT (ELF + LLLT); medical therapy (MT); and control (i.e., no treatment). Clinical examination and digital photography of the corneas were performed on days 0, 2, 7, and 14. After euthanizing the rabbits, the affected eyes were evaluated by histopathology. The clinical and histopathologic results were compared between the groups. On days 7 and 14, no significant difference in the corneal defect area was evident between the ELF, LLLT, ELF + LLLT, and MT groups. Excluding the controls, none of the study groups demonstrated a significant corneal neovascularization in both routine histopathology and immunohistochemistry for CD31. Keratocyte loss was significantly higher in the MT group than in the ELF, LLLT, and ELF + LLLT groups. Moderate to severe stromal inflammation in the LLLT group was comparable with that in the MT group and was significantly lower than that in the other groups. In conclusion, combining LLLT and ELF was not superior to ELF alone or LLLT alone in healing corneal alkali burns. However, given the lower intensity of corneal inflammation and the lower rate of keratocytes loss with LLLT, this treatment may be superior to other proposed treatment modalities for healing alkali-burned corneas. (C) 2016 Elsevier Ltd. All rights reserved.