Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach
Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach
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DOI：
10.1007/s00300-009-0714-2
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发表时间：
2010-03
期刊：
影响因子：
1.7
通讯作者：
K. Töbe;B. Meyer;V. Fuentes
中科院分区：
文献类型：
--
作者：
K. Töbe;B. Meyer;V. Fuentes
基于聚合酶链反应（PCR）的分子方法的有用性进行了调查，以确定和量化的喂养幼虫磷虾浮游动物生物在拉扎列夫海在2006年冬季。利用不同引物和探针对优势桡足类（Oithonasp.，对冬季海冰下与磷虾共生存的3种浮游生物（Ctenocalanus citer、Metridia gerlachei和Calanoides acutus）进行了定量PCR（qPCR），并对目标种和非目标种进行了种特异性检测。qPCR结果表明，磷虾幼体只以Oithonasp为食。这一结果通过对同一站点幼虫胃和肠道内容物的显微镜分析得到了证实。
The usefulness of a molecular approach based on polymerase chain reaction (PCR) was investigated to identify and quantify the feeding of larval krill on zooplankton organisms in the Lazarev Sea during winter in 2006. Different primers and probes of dominant copepod species (Oithonasp.,Ctenocalanus citer, copepodid stages ofMetridia gerlacheiandCalanoides acutus), co-occurring with larval krill under sea ice during winter, were developed for quantitative PCR (qPCR) and their species specificity was tested on target and non-target species. The qPCR results showed that larval krill were exclusively feeding onOithonasp. This result was confirmed by microscopic analysis of stomach and gut contents of larvae from the same stations.