Comparison of current methods for high-density lipoprotein cholesterol quantitation.
Comparison of current methods for high-density lipoprotein cholesterol quantitation.
复制标题
当前高密度脂蛋白胆固醇定量方法的比较。
作者：
G. R. Warnick;M C Cheung;J. J. Albers
我们比较了六种高密度脂蛋白胆固醇沉淀法和一种超速离心法，通过对d>1.063的组份中4%的操纵损失进行校正，提高了方法的准确性。为了比较的目的，减去免疫测定法测得的载脂蛋白B相关胆固醇(平均56毫克/L)在d>1.063的组份中。在65份来自男性、女性和儿童的血浆样本中，肝素-锰离子超速离心法的结果略高于超速离心法(+16 mg/L)，而肝素-锰离子超速离心法的结果略低于对照超离心法(-8 mg/L)。结果硫酸葡聚糖-镁离子(-25 mg/L)和磷钨酸盐-镁离子(-31 mg/L)法可得到约5%的低含量。肝素-钙离子法检测结果高达10%(+58 mg/L)。结果用聚乙二醇6000沉淀法测得的血药浓度低12%(-mg/L)。肝素-Mn2+和葡聚糖-硫酸镁两种方法的精密度较好，变异系数为4%，其次是磷钨酸盐-镁离子和聚乙二醇6000(CV6-7%)，肝素-钙离子最差(CV10%)。磷门-镁离子沉淀法对试剂浓度和温度的变化比肝素-锰离子或葡聚糖-硫酸镁500-镁离子沉淀法更敏感。我们得出的结论是，这些沉淀方法并不等同，但在高密度脂蛋白胆固醇定量方面引起了显著的系统差异。
We compared six precipitation methods for high-density-lipoprotein cholesterol quantitation with an ultracentrifugation method, the accuracy of which was improved by correcting for 4% manipulative loss in the d greater than 1.063 fractions. For purposes of comparison, the apoprotein B-associated cholesterol (average 56 mg/L) measured by immunoassay in the d greater than 1.063 fractions was subtracted. In 65 plasma samples from men, women, and children, a heparin-Mn2+ procedure with Mn2+ at 46 mmol/L produced results slightly higher (+16 mg/L), while results with Mn2+ at 92 mmol/L averaged slightly lower (-8 mg/L) than the comparison ultracentrifuge method. Results that were about 5% low were obtained by the dextran sulfate 500-Mg2+ (-25 mg/L) and phosphotungstate-Mg2+ (-31 mg/L) methods. A heparin-Ca2+ method produced results 10% high (+58 mg/L). Results by a polyethylene glycol-6000 precipitation method were 12% low (-64 mg/L). Precision was better with the two heparin-Mn2+ and the dextran sulfate 500-Mg2+ procedures, with CVs of 4%, intermediate with phosphotungstate-Mg2+ and polyethylene glycol-6000 (CV 6-7%), and poorest with heparin-Ca2+ (CV 10%). Precipitation by phosphotungate-Mg2+ appeared more sensitive to reagent concentration and temperature variations than either the heparin-Mn2+ or dextran sulfate 500-Mg2+ methods. We conclude that these precipitation methods are not equivalent, but give rise to significant systematic differences in high-density-lipoprotein cholesterol quantitation.