Fate of circulating renin in conscious rats.
Fate of circulating renin in conscious rats.
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清醒大鼠中循环肾素的命运。
DOI：
10.1152/ajpendo.1987.252.1.e136
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
K. Yamamoto
中科院分区：
文献类型：
--
作者：
S. Kim;H. Iwao;N. Nakamura;F. Ikemoto;K. Yamamoto
将高纯化的125i标记大鼠肾肾素（125I-renin）静脉注射到清醒大鼠体内，研究循环肾素的命运。用特异性抗大鼠肾素抗血清鉴定标记的肾素分子。假手术大鼠血浆中125i -肾素的消失表现为两种指数组分，快速组分半衰期为6.7 +/- 0.4 min，慢速组分半衰期为65.1 +/- 5.7 min。代谢清除率为11.4±1.0 ml X min-1 X kg-1。在双侧肾切除大鼠中，125i -肾素的代谢清除率降低了55%，但慢速组分的半衰期保持不变。70%的肝切除术导致代谢清除率降低54%，并延长了慢速成分的半衰期。注射125i -肾素5分钟后，大约59%和11%的125i -肾素分别积聚在肝脏和肾脏中，在稍后的时间点，125i -肾素高度集中在这些器官中。肝脏和肾脏提取物的高效液相色谱分析表明，125i -肾素在这些器官中被分解代谢。125i -肾素的胆汁排泄可忽略不计。120分钟内尿中125i -肾素的排泄量约为注射剂量的2%。我们得出结论，肝脏和肾脏都负责循环肾素的清除，肝脏的参与是主要的。
Highly purified 125I-labeled rat renal renin (125I-renin) was given intravenously to conscious rats to study the fate of circulating renin. Specific antirat renin antiserum was used to identify the labeled renin molecules. In sham-operated rats, the disappearance of 125I-renin from the plasma showed two exponential components with a half-life of 6.7 +/- 0.4 min for the rapid component and 65.1 +/- 5.7 min for the slow component. The metabolic clearance rate was 11.4 +/- 1.0 ml X min-1 X kg-1. In bilaterally nephrectomized rats, the metabolic clearance rate of 125I-renin was reduced by 55%, but the half-life of the slow component remained unchanged. Seventy percent hepatectomy caused a 54% decrement in the metabolic clearance and prolonged the half-life of the slow component. Five minutes after injection of 125I-renin, approximately 59 and 11% of the administered 125I-renin had accumulated in the liver and the kidneys, respectively, and at later time points the 125I-renin was highly concentrated in these organs. High-performance liquid chromatographic analysis of the liver and kidney extracts demonstrated that 125I-renin was catabolized by these organs. Biliary excretion of 125I-renin was negligible. Urinary excretion of 125I-renin up to 120 min was approximately 2% of the injected dose. We conclude that both the liver and the kidney are responsible for the clearance of circulating renin, with participation of the liver being predominant.