The thylakold carbonic anhydrase associated with photosystem II is the component of inorganic carbon accumulating system in cells of halo- and alkaliphilic cyanobacterium Rhabdoderma lineare
The thylakold carbonic anhydrase associated with photosystem II is the component of inorganic carbon accumulating system in cells of halo- and alkaliphilic cyanobacterium Rhabdoderma lineare
复制标题
DOI：
10.1016/j.bbabio.2006.12.006
复制
发表时间：
2007-06-01
影响因子：
4.3
通讯作者：
Pronina, Natalia A.
中科院分区：
文献类型：
--
作者：
Dudoladova, Marina V.;Kupriyanova, Elena V.;Pronina, Natalia A.
通过蛋白质印迹分析和免疫细胞化学电子显微镜研究了嗜盐和嗜碱蓝细菌线性棒状杆菌中碳酸酐酶（CA）系统的组织。据报道，糖萼中存在推定的 60 kDa 的细胞外 α-CA，在细胞周围形成紧密的鞘，并且存在两个细胞内 β-CA。我们首次证明 60 kDa 的 β-CA 是组成型表达的，并与光系统 II 的多肽（β-CA-PS II）相关。另一种 25 kDa 的可溶性 β-CA 在低碳酸氢盐培养基中诱导。后者β-CA合成的诱导伴随着细胞内无机碳池的增加，这表明该酶在CO2浓缩机制的功能中发挥着重要作用。 (c) 2007 Elsevier B.V. 保留所有权利。
The organization of carbonic anhydrase (CA) system in halo- and alkaliphilic cyanobacterium Rhabdoderma lineare was studied by Western blot analysis and immunocytochemical electron microscopy. The presence of putative extracellular alpha-CA of 60 kDa in the glycocalyx, forming a tight sheath around the cell, and of two intracellular beta-CA is reported. We show for the first time that the beta-CA of 60 kDa is expressed constitutively and associated with polypeptides of photosystem II (beta-CA-PS II). Another soluble beta-CA of 25 kDa was induced in low-bicarbonate medium. Induction of synthesis of the latter beta-CA was accompanied by an increase in the intracellular pool of inorganic carbon, which suggests an important role of this enzyme in the functioning of a CO2-concentrating mechanism. (c) 2007 Elsevier B.V. All rights reserved.