Graphite-coated paper as substrate for high sensitivity analysis in ambient surface-assisted laser desorption/ionization mass spectrometry.
Graphite-coated paper as substrate for high sensitivity analysis in ambient surface-assisted laser desorption/ionization mass spectrometry.
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DOI：
10.1021/ac300002g
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发表时间：
2012-03
影响因子：
7.4
通讯作者：
Jialing Zhang;Ze Li;Chengsen Zhang;Baosheng Feng;Zhigui Zhou;Yu Bai;Huwei Liu
中科院分区：
文献类型：
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作者：
Jialing Zhang;Ze Li;Chengsen Zhang;Baosheng Feng;Zhigui Zhou;Yu Bai;Huwei Liu
在这项工作中，建立了一种非常简单而又非常灵敏的质谱学方法，即环境表面辅助激光解吸/电离质谱仪(Ambient Saldi-MS)，只需一张石墨涂层纸作为样品介绍，就可以分析不同类型的化合物。这在简化分析过程方面提供了极大的优势。该方法使用起来相当简单，不用担心石墨的来源，也就是铅笔的品牌或类型。而且整个过程是在常压下进行的，具有在MS环境下可以发生的所有优点。这种方法提高灵敏度的主要原因是石墨作为能量传递介质，吸收光子的能量并将其释放到吸附在石墨表面的分析物。此外，还测试了三种不同的激光波长(1064、532和355 nm)，以研究解吸机理。通过51种不同化学结构的化合物的实验，证明这种新方法对检测各种有机小分子具有普遍的适用性。
In this work, an extremely simple and quite sensitive mass spectrometric method termed ambient surface-assisted laser desorption/ionization mass spectrometry (ambient SALDI-MS) was developed to analyze different kinds of compounds, just using a piece of graphite-coated paper for the sample introduction. This provides great advantage in simplifying the analysis process. The method is quite easy to use, and there is no need to worry about the source of graphite, that is, the brands or the types of pencil. And the whole process was carried out under atmospheric pressure, offering all the merits that could occur in ambient MS. The improved sensitivity of this method is mainly because of the graphite, which serves as energy-transfer medium to absorb the energy of the photons and release it to the analytes that are adsorbed on the graphite surface. Also, three different laser wavelengths (1064, 532, and 355 nm) was tested to investigate the desorption mechanism. Fifty-one compounds, with varied chemical structures, were tried to prove that this new method possessed universal applicability to detect different kinds of small organic molecules.