Long Noncoding RNA LOC100129940-N Is Upregulated in Papillary Thyroid Cancer and Promotes the Invasion and Progression
Long Noncoding RNA LOC100129940-N Is Upregulated in Papillary Thyroid Cancer and Promotes the Invasion and Progression
复制标题
长非编码RNA LOC100129940-N在甲状腺乳头状癌中表达上调并促进侵袭和进展
DOI：
10.1155/2019/7043509
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发表时间：
2019-01-01
影响因子：
2.8
通讯作者：
Guan, Hongyu
中科院分区：
文献类型：
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作者：
Choy, Manting;Guo, Yan;Guan, Hongyu
甲状腺癌是最常见的内分泌恶性肿瘤，近几十年来在世界范围内发病率迅速上升。乳头状甲状腺癌（PTC）是所有甲状腺癌中最常见的类型。该疾病的分子机制仍需进一步研究。长链非编码rna （lncRNAs）是一类长度超过200个核苷酸的非编码rna (ncRNAs)，在包括PTC在内的恶性疾病中异常表达。在这里，我们鉴定了LOC100129940的一个新的异构体，并将其命名为LOC100129940- n。我们发现LOC100129940-N在PTC中的表达水平升高，表明LOC100129940-N可能参与了PTC的发生和进展。此外，我们的研究结果表明，LOC100129940-N的过表达促进了PTC细胞的增殖、侵袭和迁移，而LOC100129940-N的沉默则抑制了PTC细胞的增殖、侵袭和迁移。从机制上讲，LOC100129940-N在激活Wnt/β-catenin信号通路和上调下游靶基因中发挥了重要作用。综上所述，我们证明LOC100129940-N促进Wnt/β-catenin信号的激活，进而调控下游靶基因，从而促进PTC的侵袭和进展。
Thyroid cancer is the most common endocrine malignancy, and its incidence has increased rapidly in recent decades worldwide. Papillary thyroid cancer (PTC) is the most common type of all thyroid cancers. The molecular mechanisms underlying the disease still need to be further investigated. Long noncoding RNAs (lncRNAs), a class of noncoding RNAs (ncRNAs) longer than 200 nucleotides, are aberrantly expressed in malignant diseases, including PTC. Here, we identified a novel isoform of LOC100129940 and designated it as LOC100129940-N. We demonstrated that the expression level of LOC100129940-N was elevated in PTC, indicating that LOC100129940-N may be involved in PTC development and progression. Moreover, our results showed that overexpression of LOC100129940-N promoted, whereas silencing of LOC100129940-N suppressed, PTC cell proliferation, invasion, and migration. Mechanistically, LOC100129940-N played an important role in activating Wnt/β-catenin signaling and upregulating downstream target genes. Taken together, we demonstrate that LOC100129940-N promotes the activation of Wnt/β-catenin signaling, which in turn regulates the downstream target genes, thereby enhancing invasion and progression of PTC.