Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation
Microwave Monitoring of Single Cell Monocytes Subjected to Electroporation
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DOI：
10.1109/tmtt.2017.2653776
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发表时间：
2017-09-01
影响因子：
4.3
通讯作者：
Grenier, Katia
中科院分区：
文献类型：
--
作者：
Tamra, Amar;Dubuc, David;Grenier, Katia
本文介绍了利用微波介电频谱对电穿孔单个电池进行监测的方法。实验结果首先显示了两种细胞状态之间的明显区别：活细胞和受化学处理(皂苷)影响的细胞。它还指出，在检测可逆电穿孔和不可逆电穿孔这两种类型的电穿孔时，与生物标准技术有很高的相关性(R-2和0.94)。研制的微流控和基于微波的传感器显示，经过不可逆电穿孔处理的单个电池的电容和导电对比度降低，表明细胞水平上的损伤，而可逆电穿孔处理的电池表现出与未处理细胞相似的介电响应。这一结果与生物学研究中经常使用的结果相对应。更有趣的是，通过实验研究了电穿孔诱导细胞损伤的动力学，这使得微波介电光谱成为研究细胞电穿孔的一种有吸引力的技术。
This paper presents the monitoring of single cells subjected to electroporation using microwave dielectric spectroscopy. The experimental results showed first a clear distinction between two cell states: viable cells and affected ones by a chemical treatment (Saponin). It also pointed out a high correlation (R-2 > 0.94) with biological standard techniques in detecting the two types of electroporation: the reversible and irreversible ones. The developed microfluidic and microwavebased sensor exposes a decrease in the capacitive and conductive contrasts of the investigated single cells treated by irreversible electroporation indicating damages at the cellular level, while cells under reversible electroporation present a similar dielectric response to that of the nontreated cells. This result corresponds to results frequently employed in biological studies. More interestingly, a study of the kinetics of the cell's damage induction over time, by electroporation, has been experimentally done, which makes microwave dielectric spectroscopy an attractive technique for cell's electroporation researches.