Coronavirus RNA synthesis takes place within membrane-bound sites
Coronavirus RNA synthesis takes place within membrane-bound sites
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冠状病毒 RNA 合成发生在膜结合位点内
DOI：
10.1101/2021.11.04.467246
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Doyle N
中科院分区：
文献类型：
--
作者：
Doyle N
传染性支气管炎病毒（IBV），一种γ冠状病毒，是家禽业的重要经济病毒，也是鸡的重要福利问题。与所有正链RNA病毒一样，IBV感染引起宿主细胞胞内膜重排以形成复制细胞器。复制细胞器的形成是病毒生命周期中高度保守和重要的步骤。在这里，我们调查的病毒RNA合成的本地化和复制细胞器在宿主细胞中的链接。我们已经证明，病毒RNA合成的位点和病毒相关的dsRNA是相互关联的，并且重要的是，它们位于细胞内的膜结合区室中。我们还表明，在感染早期产生的一些病毒RNA在整个感染过程中仍然存在于这些膜内，而一部分则被运输到细胞质中。重要的是，我们证明了所有四种冠状病毒属的保守性，包括SARS-CoV-2。为了有效地找到控制它们的方法，必须更多地了解这些病毒的复制。
Infectious bronchitis virus (IBV), a gammacoronavirus, is an economically important virus to the poultry industry, as well as a significant welfare issue for chickens. As for all positive strand RNA viruses, IBV infection causes rearrangements of the host cell intracellular membranes to form replication organelles. Replication organelle formation is a highly conserved and vital step in the viral life cycle. Here, we investigate the localization of viral RNA synthesis and the link with replication organelles in host cells. We have shown that sites of viral RNA synthesis and virus-related dsRNA are associated with one another and, significantly, that they are located within a membrane-bound compartment within the cell. We have also shown that some viral RNA produced early in infection remains within these membranes throughout infection, while a proportion is trafficked to the cytoplasm. Importantly, we demonstrate conservation across all four coronavirus genera, including SARS-CoV-2. Understanding more about the replication of these viruses is imperative in order to effectively find ways to control them.