Dendronized Semiconducting Polymer as Photothermal Nanocarrier for Remote Activation of Gene Expression
Dendronized Semiconducting Polymer as Photothermal Nanocarrier for Remote Activation of Gene Expression
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DOI：
10.1002/anie.201705543
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发表时间：
2017-07-24
影响因子：
16.6
通讯作者：
Pu, Kanyi
中科院分区：
文献类型：
--
作者：
Lyu, Yan;Cui, Dong;Pu, Kanyi
为了开发创新药物，需要对转基因系统进行监管。然而，对基因表达的非侵入性远程控制很少被开发出来，仍然具有挑战性。在此，我们合成了一种近红外(NIR)吸收的树枝状半导体聚合物(DSP)，并将其用作光热纳米载体，不仅可以有效地传递基因，还可以与热诱导启动子一起在时空上控制基因的表达。DSP在808 nm处有很高的光热转换效率(44.2%)，可以将近红外光快速转化为热信号，从而激活细胞内的转录。这种由DSP介导的远程激活可以迅速而安全地导致活细胞和小鼠中蛋白质表达水平分别增加25倍和4.5倍。因此，这项研究为按需治疗性转基因剂量的转基因系统提供了一种有前途的光学调控方法。
Regulation of transgene systems is needed to develop innovative medicines. However, noninvasive remote control of gene expression has been rarely developed and remains challenging. We herein synthesize a near-infrared (NIR) absorbing dendronized semiconducting polymer (DSP) and utilize it as a photothermal nanocarrier not only to efficiently deliver genes but also to spatiotemporally control gene expression in conjunction with heat-inducible promoter. DSP has a high photothermal conversion efficiency (44.2%) at 808 nm, permitting fast transduction of NIR light into thermal signals for intracellular activation of transcription. Such a DSP-mediated remote activation can rapidly and safely result in 25-and 4.5-fold increases in the expression levels of proteins in living cells and mice, respectively. This study thus provides a promising approach to optically regulate transgene systems for on-demand therapeutic transgene dosing.