TRANSCRIPTION AND REPLICATION OF 8 RNA SEGMENTS OF INFLUENZA-VIRUS
TRANSCRIPTION AND REPLICATION OF 8 RNA SEGMENTS OF INFLUENZA-VIRUS
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DOI：
10.1016/0042-6822(85)90423-4
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发表时间：
1985-01-01
期刊：
影响因子：
3.7
通讯作者：
ISHIHAMA, A
中科院分区：
文献类型：
--
作者：
ENAMI, M;FUKUDA, R;ISHIHAMA, A
以单链c[互补]DNA为杂交探针，建立了流感病毒全部8个基因组片段的正链和负链RNA定量体系，并用于流感病毒WSN感染的MDBK细胞中多种RNA的测定。正链RNA的合成速率和积累水平在8个RNA片段之间存在较大差异，并受时间控制。以相似的速率协调合成了8个负极性的v[病毒]RNA分子。新合成的正链RNA迅速进入细胞质，特别是在病毒感染的早期，但vRNA积累在细胞核中，直到感染晚期。目前的数据支持合成的差异调节和正链和负链RNA之间的分离运输。
A novel quantitation system of both plus- and minus-strand RNA for all 8 genome segments of influenza virus was developed using single-strand c[complementary]DNA as the probes for hybridization, and employed for the measurement of various RNA species in influenza virus WSN-infected MDBK cells. The synthesis rate and accumulation level of plus-strand RNA differed considerably among 8 RNA segments and were under temporal control. Eight v[viral]RNA molecules of minus polarity were synthesized coordinately at similar rates. Newly synthesized plus-strand RNA were rapidly transported into the cytoplasm, particularly during the early phase of virus infection, but vRNA accumulated in the nuclei until the late infection phase. The present data supported the differential regulation of synthesis and the separate transport between plus- and minus-strand RNA.