A Quantitative Analysis of Drug Loading Efficiency and Real-Time Drug Release in ZrO2 Nanoparticles with Energy Spectrum Computed Tomography
A Quantitative Analysis of Drug Loading Efficiency and Real-Time Drug Release in ZrO2 Nanoparticles with Energy Spectrum Computed Tomography
复制标题
利用能谱计算机断层扫描定量分析 ZrO2 纳米粒子的载药效率和实时药物释放
DOI：
10.1166/jbn.2021.3051
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发表时间：
2021
影响因子：
2.9
通讯作者：
Liu Yi
中科院分区：
文献类型：
--
作者：
Ji Wanying;Qi Xun;Li Hui;Zhang Yanyan;Sun Yang;Wang Jun;Xu Ke;Liu Yi
能谱计算机层析成像(CT)可以定量测定体内和体外物质的浓度。在本研究中，我们设计了一种载多柔比星(DOX)的二氧化锆纳米粒(DOX@ZrO2 NPs)单壳体系作为一种新型的化疗药物释放系统。用能谱CT监测DOX@ZrO2纳米粒在组织中的浓度，计算DOX从纳米粒中的释放量。ZrO2纳米颗粒的梯度浓度与基材含量的标准曲线符合对数方程。将细胞与200mLDOX@μ_2孵育不同时间。能谱CT检测细胞内药物浓度与细胞内化疗药物浓度呈显著正相关(r=0.98，P&t；0.05)。这些数据表明，能谱CT是实时监测NPs在局部组织中的传输和NP有效载荷释放的可靠手段。这一发现可以提高临床影像的准确性，促进NPs的治疗应用。无临床试验注册：本实验未涉及动物和人体材料。
Energy spectrum computed tomography (CT) can quantify the concentrations of substances in vitro and in vivo. In this study, we designed a single-shell system of doxorubicin (DOX) loaded in zirconium dioxide nanoparticles (DOX@ZrO2 NPs) as a novel chemotherapy drug delivery system. The concentration of DOX@ZrO2 NPs in tissue was monitored with energy spectrum CT to calculate the release of DOX from the NPs. The standard curve of the gradient concentrations of ZrO2 NPs and base material content fit a logarithmic equation. HepG2 cells were incubated with 200 μmL DOX@ZrO2 for different times. The concentration in the cells detected with energy spectrum CT correlated strongly with the concentration of chemotherapeutics in the cells (r = 0.98, P < 0.05). The data indicate that energy spectrum CT is a reliable means of real-time monitoring of the transport of NPs and release of the NP payload in local tissue. The finding could improve the accuracy of clinical imaging and promote the therapeutic use of NPs. Free of clinical trial registeration: There were no animal and human materials involved in this experiment.