Multibranch activity in basal and tuft dendrites during firing of layer 5 cortical neurons in vivo
Multibranch activity in basal and tuft dendrites during firing of layer 5 cortical neurons in vivo
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DOI：
10.1073/pnas.1312599110
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发表时间：
2013-08-13
影响因子：
11.1
通讯作者：
Konnerth, Arthur
中科院分区：
文献类型：
--
作者：
Hill, Daniel N.;Varga, Zsuzsanna;Konnerth, Arthur
第5层锥体神经元处理来自多个皮层层的信息，提供皮层的主要输出。由于技术上的限制，目前尚不清楚这些细胞是如何整合分布广泛的突触输入的，这些突触输入位于遥远的基部和丛状树突上。在这里，我们获得了来自第5层运动皮层神经元整个树突状场的体内双光子钙成像记录。我们证明，在阈下活动期间，基底树突和丛状树突表现出空间定位的小幅度钙瞬态，反映传入突触输入。在动作电位放电过程中，基底树突中的钙信号与spike活动呈线性相关，而簇状树突中的钙信号则不可靠。然而，在两个树突腔室中，与尖峰相关的钙信号均匀分布在所有分支中。因此，我们的数据支持一个广泛的、多分支整合的模型，该模型直接影响基部树突的输出信号，而丛的输出信号仅部分贡献。
Layer 5 pyramidal neurons process information from multiple cortical layers to provide a major output of cortex. Because of technical limitations it has remained unclear how these cells integrate widespread synaptic inputs located in distantly separated basal and tuft dendrites. Here, we obtained in vivo two-photon calcium imaging recordings from the entire dendritic field of layer 5 motor cortex neurons. We demonstrate that during subthreshold activity, basal and tuft dendrites exhibit spatially localized, small-amplitude calcium transients reflecting afferent synaptic inputs. During action potential firing, calcium signals in basal dendrites are linearly related to spike activity, whereas calcium signals in the tuft occur unreliably. However, in both dendritic compartments, spike-associated calcium signals were uniformly distributed throughout all branches. Thus, our data support a model of widespread, multibranch integration with a direct impact by basal dendrites and only a partial contribution on output signaling by the tuft.