IMMUNOENHANCING EFFECT OF FLAVONOID COMPOUNDS ON LYMPHOCYTE-PROLIFERATION AND IMMUNOGLOBULIN-SYNTHESIS
IMMUNOENHANCING EFFECT OF FLAVONOID COMPOUNDS ON LYMPHOCYTE-PROLIFERATION AND IMMUNOGLOBULIN-SYNTHESIS
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DOI：
10.1016/0192-0561(84)90018-3
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
BERG, PA
中科院分区：
文献类型：
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作者：
BRATTIG, NW;DIAO, GJ;BERG, PA
黄酮类化合物是亲脂性试剂，其可以与膜脂质相互作用并且可以影响免疫细胞的反应性。研究了Cianidanol（（+）-儿茶素）、O-甲基-衍生物（+）-3-甲氧基-5，7，3“，4”-四羟基黄烷和棕榈酰-衍生物（+）-3-棕榈酰-5，7，3“，4”-四羟基黄烷是否影响[人] T和B细胞功能。还研究了泛醌50的免疫调节性质。分别使用已知抑制或增强免疫应答的环孢菌素A和肌苷作为对照。在不同浓度的试剂存在下，测定了来自健康个体的外周血单个核细胞的体外自发、抗原和有丝分裂原诱导的增殖以及IG合成。所有黄酮类化合物和泛醌50均能显著促进淋巴细胞自发转化（P < 0.05 ~< 0.01），但对抗原、同种抗原和丝裂原诱导的淋巴细胞增殖反应无明显影响。只有Cianidanol和O-甲基衍生物显著（P < 0.05 ~< 0.01）促进自发性，美洲商陆丝裂原和金黄色葡萄球菌科万I诱导IG合成，而棕榈酰衍生物和泛醌50对B细胞功能的影响很小。S.金黄色葡萄球菌诱导的IG产生既不被肌苷增加也不被环孢菌素A抑制。尤其是西尼丹醇和O-甲基衍生物可对T和B细胞功能发挥免疫增强作用。
Flavonoid compounds are lipophilic agents which can interact with membrane lipids and may affect responsiveness of immune cells. It was studied whether cianidanol ((+)-catechin), the O-methyl-derivative (+)-3-methoxy-5,7,3'',4''-tetrahydroxyflavan and palmitoyl-derivative (+)-3-palmitoyl-5,7,3'',4''-tetrahydroxyflavan influence [human] T and B cell functions. Immunomodulatory property of ubiquinone 50 was also investigated. Cyclosporin A and inosine which are known to inhibit or enhance immune responses, respectively, were used as controls. The in vitro spontaneous, antigen and mitogen induced proliferation as well as Ig synthesis of peripheral blood mononuclear cells from healthy individuals was determined in the presence of different concentrations of the agents. All flavonoid compounds and ubiquinone 50 significantly increased (P < 0.05-< 0.01) the spontaneous lymphocyte transformation but hardly affected antigen, alloantigen and mitogen induced proliferative response. Only cianidanol and O-methyl-derivative enhanced significantly (P < 0.05-< 0.01) spontaneous, pokeweed mitogen and Staphylococcus aureus Cowan I induced Ig synthesis while the palmitoyl-derivative and ubiquinone 50 had only minor influence on B cell function. S. aureus induced Ig production was neither increased by inosine nor suppressed by cyclosporin A. Especially cianidanol and the O-methyl-derivative can exert an immunoenhancing effect on T and B cell functions.