NEUROCHEMICAL PHENOTYPE OF CORTICOCORTICAL CONNECTIONS IN THE MACAQUE MONKEY - QUANTITATIVE-ANALYSIS OF A SUBSET OF NEUROFILAMENT PROTEIN-IMMUNOREACTIVE PROJECTION NEURONS IN FRONTAL, PARIETAL, TEMPORAL, AND CINGULATE CORTICES
NEUROCHEMICAL PHENOTYPE OF CORTICOCORTICAL CONNECTIONS IN THE MACAQUE MONKEY - QUANTITATIVE-ANALYSIS OF A SUBSET OF NEUROFILAMENT PROTEIN-IMMUNOREACTIVE PROJECTION NEURONS IN FRONTAL, PARIETAL, TEMPORAL, AND CINGULATE CORTICES
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DOI：
10.1002/cne.903620107
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发表时间：
1995-11-06
影响因子：
2.5
通讯作者：
MORRISON, JH
中科院分区：
文献类型：
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作者：
HOF, PR;NIMCHINSKY, EA;MORRISON, JH
提供不同类型皮质连接的神经元亚群的神经化学特征仅被部分确定。近年来，几种细胞骨架蛋白已成为区分灵长类大脑皮层锥体神经元亚群的可靠标记。特别是，以前使用非磷酸化神经丝蛋白抗体(SMI-32)的研究表明，在灵长类大脑皮层锥体细胞亚群的分布中，区域和板层特异性程度一致。在猕猴中，神经丝蛋白免疫反应神经元的密度在皮质通路上是不同的。在目前的研究中，我们使用抗体SMI-32来进一步检查和量化皮质投射神经元的子集在一系列同侧皮质长通路中的分布，并与皮质、连合和边缘短连接进行比较。结果表明，连接额叶、顶叶和颞叶新皮质的长联结通路含有大量富含神经丝蛋白的锥体神经元(45%-90%)，而皮质、穹隆和边缘短路的锥体神经元数量较少(4%-35%)。这些数据表明，不同类型的皮质连接具有高度特异性的神经元亚群的不同表示，这些神经元亚群具有共同的神经化学特征，从而决定了形态和分子异质性的区域和板层皮质模式。皮质环路之间神经元化学表型的这些差异可能对皮质加工有相当大的影响，并可能与每条皮质通路所支持的综合功能的类型直接相关。最后，值得注意的是，神经丝蛋白免疫反应神经元在阿尔茨海默病的过程中受到极大的影响。目前的结果支持这一假设，即神经丝蛋白可能与选择性神经元脆弱性的发展以及随后的皮质通路中断有关，这些通路导致在年龄相关性痴呆中常见的严重认知功能障碍。(C)1995年Wiley-Liss公司
The neurochemical characteristics of the neuronal subsets that furnish different types of corticocortical connections have been only partially determined. In recent years, several cytoskeletal proteins have emerged as reliable markers to distinguish subsets of pyramidal neurons in the cerebral cortex of primates. In particular, previous studies using an antibody to nonphosphorylated neurofilament protein (SMI-32) have revealed a consistent degree of regional and laminar specificity in the distribution of a subpopulation of pyramidal cells in the primate cerebral cortex. The density of neurofilament protein-immunoreactive neurons was shown to vary across corticocortical pathways in macaque monkeys. In the present study, we have used the antibody SMI-32 to examine further and to quantify the distribution of a subset of corticocortically projecting neurons in a series of long ipsilateral corticocortical pathways in comparison to short corticocortical, commissural, and limbic connections. The results demonstrate that the long association pathways interconnecting the frontal, parietal, and temporal neocortex have a high representation of neurofilament protein-enriched pyramidal neurons (45-90%), whereas short corticocortical, callosal, and limbic pathways are characterized by much lower numbers of such neurons (4-35%). These data suggest that different types of corticocortical connections have differential representation of highly specific neuronal subsets that share common neurochemical characteristics, thereby determining regional and laminar cortical patterns of morphological and molecular heterogeneity. These differences in neuronal neurochemical phenotype among corticocortical circuits may have considerable influence on cortical processing and may be directly related to the type of integrative function subserved by each cortical pathway. Finally, it is worth noting that neurofilament protein-immunoreactive neurons are dramatically affected in the course of Alzheimer's disease. The present results support the hypothesis that neurofilament protein may be crucially linked to the development of selective neuronal vulnerability and subsequent disruption of corticocortical pathways that lead to the severe impairment of cognitive function commonly observed in age-related dementing disorders. (C) 1995 Wiley-Liss, Inc.