Developmental Increase in Kisspeptin-54 Release in Vivo Is Independent of the Pubertal Increase in Estradiol in Female Rhesus Monkeys (Macaca mulatta)
Developmental Increase in Kisspeptin-54 Release in Vivo Is Independent of the Pubertal Increase in Estradiol in Female Rhesus Monkeys (Macaca mulatta)
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DOI：
10.1210/en.2011-1701
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发表时间：
2012-04-01
期刊：
影响因子：
4.8
通讯作者：
Terasawa, Ei
中科院分区：
文献类型：
--
作者：
Guerriero, Kathryn A.;Keen, Kim L.;Terasawa, Ei
Kisspeptin（KP）信号通路被认为是青春期发育的重要调节因子。在这项研究中，以确定KP在青春期的作用，我们评估了KP-54的体内释放模式从基底下丘脑/茎正中隆起在青春期前和青春期卵巢完整的雌性恒河猴。我们发现，平均KP-54释放，脉冲频率和脉冲幅度的发育增加，这与我们以前报道的GnRH释放的发育变化平行。此外，在青春期开始后，KP-54释放的夜间增加变得突出。由于青春期GnRH释放的增加独立于青春期循环性腺类固醇的增加，我们进一步研究了卵巢切除术（OVX）是否改变了KP-54的释放模式。结果表明，OVX在青春期的猴子提高平均KP-54的释放和脉冲幅度，但不脉冲频率，而OVX没有改变KP-54的释放模式在青春期前的猴子。在OVX青春期猴中，Eglutamine替代抑制平均KP-54释放和脉冲幅度，但不抑制脉冲频率。在OVX青春期前的猴子中，雌二醇替代没有改变KP-54的释放模式。总的来说，这些结果表明，青春期增加KP释放发生独立的青春期增加循环雌二醇。然而，青春期KP释放的增加不太可能导致青春期GnRH释放增加。相反，在青春期开始后，KP释放的增加有助于进一步增加青春期进展期间的GnRH释放。（内分泌学153：1887-1897，2012）
Kisspeptin (KP) signaling has been proposed as an important regulator in the mechanism of puberty. In this study, to determine the role of KP in puberty, we assessed the in vivo release pattern of KP-54 from the basal hypothalamus/stalk-median eminence in prepubertal and pubertal ovarian-intact female rhesus monkeys. We found that there was a developmental increase in mean KP-54 release, pulse frequency, and pulse amplitude, which is parallel to the developmental changes in GnRH release that we previously reported. Moreover, a nocturnal increase in KP-54 release becomes prominent after the onset of puberty. Because the pubertal increase in GnRH release occurs independent of the pubertal increase in circulating gonadal steroids, we further examined whether ovariectomy (OVX) modifies the release pattern of KP-54. Results show that OVX in pubertal monkeys enhanced mean KP-54 release and pulse amplitude but not pulse frequency, whereas OVX did not alter the release pattern of KP-54 in prepubertal monkeys. Estradiol replacement in OVX pubertal monkeys suppressed mean KP-54 release and pulse amplitude but not pulse frequency. Estradiol replacement in OVX prepubertal monkeys did not alter the KP-54 release pattern. Collectively these results suggest that the pubertal increase in KP release occurs independent of the pubertal increase in circulating estradiol. Nevertheless, the pubertal increase in KP release is not likely responsible for the initiation of the pubertal increase in GnRH release. Rather, after puberty onset, the increase in KP release contributes to further increase GnRH release during the progression of puberty. (Endocrinology 153: 1887-1897, 2012)