Characterization of prostate cell types by CD cell surface molecules
Characterization of prostate cell types by CD cell surface molecules
复制标题
DOI：
10.1016/s0002-9440(10)64346-5
复制
发表时间：
2002-01-01
影响因子：
6
通讯作者：
True, LD
中科院分区：
文献类型：
--
作者：
Liu, AY;True, LD
一组针对淋巴细胞表面分子的单抗，即簇标(CD)抗原，被用来区分前列腺的组成细胞类型。腔内分泌上皮、基底上皮、纤维肌肉间质、神经鞘和内皮细胞表达细胞表面分子的独特补体，免疫组织化学使用152种商用抗体对其进行鉴定。许多CD抗体对前列腺中的淋巴细胞群进行了染色。这些淋巴细胞群被分成稀有、中等和高度三个丰度等级。这些分子中的一些由多种细胞类型表达，包括实质细胞和淋巴样细胞；另一些仅由一种细胞类型表达。CD表达的独特模式与前列腺腔细胞的表达模式最相似，也是Gleason Score 6小系列前列腺癌的特征。CD分子的细胞类型特异性增加了利用激光捕获显微解剖和流式细胞术等技术分离特定细胞群进行细胞特异性分子研究的可能性。
A set of monoclonal antibodies raised against lymphocyte cell surface molecules, the cluster designation (CD) antigens, was used to distinguish the constituent cell types of the prostate. The luminal secretory epithelial, basal epithelial, fibromuscular stromal, nerve sheath, and endothelial cells express distinctive complements of cell surface molecules that were identified by immunohistochemistry using 152 commercially available antibodies. Many of the CD antibodies stained lymphocyte populations in the prostate. These lymphocyte populations were grouped into abundance classes of rare, moderate, and high. Some of these molecules are expressed by multiple cell types, both parenchymal and lymphoid; others are expressed by only one cell type. Distinctive patterns of CD expression, which are most similar to the expression pattern of prostate luminal cells, also characterize a small series of Gleason score 6 prostate cancers. The cell-type specificity of CD molecules increases the prospect of Isolating specific cell populations, using such techniques as laser capture microdissection and flow cytometry, for cell-specific molecular studies.