Adenovirus terminal protein protects single stranded DNA from digestion by a cellular exonuclease.
Adenovirus terminal protein protects single stranded DNA from digestion by a cellular exonuclease.
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腺病毒末端蛋白保护单链 DNA 免受细胞核酸外切酶的消化。
DOI：
10.1093/nar/8.3.543
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发表时间：
1980
影响因子：
14.9
通讯作者：
Berk,AJ
中科院分区：
文献类型：
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作者：
Dunsworth-Browne,M;Schell,RE;Berk,AJ
腺病毒5型DNA-蛋白复合物是从病毒体中分离出来的双链DNA分子，每条链的5′端与功能未知的病毒体蛋白共价连接。DNA-蛋白质复合物可用E.大肠杆菌外切核酸酶III产生类似于DNA复制中间体的小分子，因为它们含有长的单链区域，其5′末端与末端蛋白结合。链霉蛋白酶消化的腺病毒5 DNA的感染性被核酸外切酶III消化大大降低。然而，当从每条链的3′端除去至少2400个核苷酸时，DNA-蛋白质复合物的感染性没有显著改变。这表明末端蛋白保护5′端单链区域不被细胞核酸外切酶消化。用核酸外切酶III消化从在基因组的左侧4%中具有突变的宿主范围突变体制备的DNA-蛋白质复合物，与包含基因组的左侧8%的野生型限制性片段杂交，并转染到HeLa细胞中。以高频率回收具有野生型表型的病毒。
Adenovirus 5 DNA-protein complex is isolated from virions as a duplex DNA molecule covalently attad by the 5′ terminl of each strand to virion protein of unknown function. The DNA-protein complex can be digested withE. coliexonuclease III to generate nolecules analogous to DNA replication intermediates in that they contain long single stranded regions ending in 5′ termini bound to terminal protein. The infectivity of pronase digested Adenovirus 5 DNA is greatly diminished by exonuclease III digestion. However, the infectivity of the DNA-protien complex is not significantly altered when up to at least 2400 nucelcotides are removed frcm the 3′ ends of each strand. This indicates that the terminal protein protects 5′ terminated single stranded regions from digestion by a cellular exonuclease. DNA-protein complex prepared from a host range mutant with a mutation in the left 4% of the genome was digested with exonuclease III, hybridized to a wild type restriction fragnent comprising the left 8% of the genome, and transfected into HeLa cells. Virus with wild type phenotype was recovered at high frequency.
R.G.Duggleby：“酶学方法”
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