Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
复制标题
DOI：
10.1002/jbio.201500163
复制
发表时间：
2016-03-01
影响因子：
2.8
通讯作者：
Yakovlev, Vladislav V.
中科院分区：
文献类型：
--
作者：
Meng, Zhaokai;Lopez, Sandra C. Bustamante;Yakovlev, Vladislav V.
布里渊显微光谱技术是一种强有力的无创光学成像技术。特别是，布里渊显微光谱学独特地允许以微观空间分辨率评估样品的机械特性。无背景布里渊显微光谱学的最新进展使得在不显著降低数据质量的情况下对散射样品进行成像成为可能。然而，由于信号强度有限，迄今为止从未进行过蜂窝和亚蜂窝水平的测量。在这份报告中，通过采用我们最近优化的基于VIPA的布里渊光谱仪，我们探测了单个红细胞的微观粘弹性。这些测量通过使用拉曼显微光谱的化学特定测量来补充。
Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular-and subcellular-level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA-based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy.