Anti-idiotypic antibodies induce neutralizing antibodies to rabies virus glycoprotein.
Anti-idiotypic antibodies induce neutralizing antibodies to rabies virus glycoprotein.
复制标题
抗独特型抗体诱导针对狂犬病病毒糖蛋白的中和抗体。
DOI：
10.1128/jvi.48.3.660-666.1983
复制
发表时间：
1983
影响因子：
5.4
通讯作者：
Koprowski,H
中科院分区：
文献类型：
--
作者：
Reagan,KJ;Wunner,WH;Wiktor,TJ;Koprowski,H
针对狂犬病病毒糖蛋白特异性的五种鼠单克隆抗体（mAb）制备兔抗独特型抗体（α Id Ab）。四个单克隆抗体是针对三个已知的，类型特异性，中和位点的糖蛋白，和其他单克隆抗体是针对一个地形的非特征性，非中和表位。α Id Ab与异源mAb之间不存在显著交叉反应性，表明α Id Ab对独特可变区决定簇具有高度特异性。狂犬病病毒可溶性糖蛋白可抑制5个α Id Ab中的3个与其同源mAb的结合，表明α Id Ab具有与mAb的抗原结合位点相似或相邻的亚群。注射到小鼠中的五种α Id Ab中的两种引起特异性病毒中和抗体应答。解释由α Id Ab诱导病毒中和抗体的机制进行了讨论。
Rabbit anti-idiotypic antibodies (alpha Id Ab) were prepared against five murine monoclonal antibodies (mAb) specific for the rabies virus glycoprotein. Four of the mAb were directed against three known, type-specific, neutralizing sites on the glycoprotein, and the other mAb was directed against a topographically uncharacterized, nonneutralizing epitope. An absence of significant cross-reactivity among the alpha Id Ab for heterologous mAb suggested that the alpha Id Ab were highly specific for unique variable region determinants. The binding of three of the five alpha Id Ab to their homologous mAb could be inhibited by rabies virus-soluble glycoprotein, suggesting that the alpha Id Ab possessed subpopulations similar or adjacent to the antigen-binding site of the mAb. Two of the five alpha Id Ab injected into mice elicited a specific virus-neutralizing antibody response. Mechanisms to account for the induction of the virus-neutralizing antibody by alpha Id Ab are discussed.