PHYSIOLOGY AND MORPHOLOGY OF SUBSTANTIA GELATINOSA NEURONS INTRACELLULARLY STAINED WITH HORSERADISH-PEROXIDASE
PHYSIOLOGY AND MORPHOLOGY OF SUBSTANTIA GELATINOSA NEURONS INTRACELLULARLY STAINED WITH HORSERADISH-PEROXIDASE
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DOI：
10.1002/cne.901940407
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发表时间：
1980-01-01
影响因子：
2.5
通讯作者：
DUBNER, R
中科院分区：
文献类型：
--
作者：
BENNETT, GJ;ABDELMOUMENE, M;DUBNER, R
在猫的Rexed的层II的神经元的生理特征与自然和电刺激施加到他们的皮肤感受野。然后用辣根过氧化物酶对神经元进行细胞内染色。伤害性神经元对温和的机械刺激无反应。大多数只对组织损伤刺激作出反应。有些人也对中等重的压力作出反应，但这些人对有害刺激的反应是放电频率增加。宽动态范围神经元对温和的机械刺激和组织损伤刺激都有反应。低阈值机械感受神经元只对非机械刺激有反应。一些低阈值的机械感受性神经元由无髓轴突的初级传入神经支配。除了那些低阈值的mechanoreceptive神经元与输入无髓传入，第二层神经元的初级传入神经支配的模式是类似的神经支配的模式，已被发现的神经元在第一和IV-V层。所有，但2的22个神经元被认为是2个一般的形态类型。柄细胞的胞体沿着于第II层的表面边缘。大多数树突向腹侧行进，同时向吻尾侧伸展。这使得它们的树枝状乔木呈扇形。有柄细胞轴突主要在第I层分叉。胰岛细胞胞体遍布第II层。大多数树突向吻尾方向走行。其树突状结构呈圆柱状，长轴与脊髓长轴平行。胰岛细胞轴突在其树突状领土附近，在第二层。柄细胞和胰岛细胞的树突状乔木主要包含在浅1/3的层II（IIa层）的伤害性特异性或广泛的动态范围的神经元。树突状细胞主要位于第二层深2/3（第二b层）的胰岛细胞均为低阈值机械感受性神经元。显然，IIa和IIb层具有不同的功能作用，柄细胞和胰岛细胞是浅表背角神经回路的独立和不同的组成部分。
Neurons in Rexed''s layer II in cats were physiologically characterized with natural and electrical stimuli applied to their cutaneous receptive fields. The neurons were then intracellularly stained with horseradish peroxidase. Three general patterns of physiological responses were found. Nociceptive specific neurons did not respond to gentle mechanical stimulation. Most responded exclusively to tissue-damaging stimuli. Some also responded to moderately heavy pressure, but these responded to noxious stimuli with an increased discharge frequency. Wide dynamic range neurons responded to both gentle mechanical stimulation and to tissue-damaging stimulation. Low-threshold mechanoreceptive neurons responded only to gentile mechanical stimulation. Some of the low-threshold mechanoreceptive neurons were innervated by primary afferents with unmyelinated axons. Excepting those low-threshold mechanoreceptive neurons with input from unmyelinated afferents, the patterns of primary afferent innervation of layer II neurons were similar to the patterns of innervation that have been found for neurons in layers I and IV-V. All but 2 of the 22 neurons found were recognized as being of 2 general morphological types. Stalked cells had their perikarya situated along the superficial border of layer II. Most of their dendrites traveled ventrally while spreading out rostrocaudally. This gave their dendritic arbors a fan-like shape. Stalked cell axons arborized largely in layer I. Islet cell perikarya were found throughout layer II. Most of their dendrites traveled rostracaudally. Their dendritic arbors were shaped like cylinders with their long axes parallel to the long axis of the spinal cord. Islet cell axons arborized in the immediate vicinity of their dendritic territories, within layer II. Stalked cells and those islet cells whose dendritic arbors were largely contained within the superficial 1/3 of layer II (layer IIa) were either nociceptive specific or wide dynamic range neurons. The islet cells whose dendritic arbors were largely within the deeper 2/3 of layer II (layer IIb) were all low-threshold mechanoreceptive neurons. Apparently layers IIa and IIb have different functional roles and stalked cells and islet cells are separate and distinct components of the neural circuitry of the superficial dorsal horn.