Characterisation of isolated α-synuclein filaments from substantia nigra of Parkinson's disease brain
Characterisation of isolated α-synuclein filaments from substantia nigra of Parkinson's disease brain
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DOI：
10.1016/s0304-3940(00)01440-3
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发表时间：
2000-10-06
影响因子：
2.5
通讯作者：
Goedert, M
中科院分区：
文献类型：
--
作者：
Crowther, RA;Daniel, SE;Goedert, M
帕金森病、路易体痴呆和多系统萎缩的定义性神经病理学沉积物对α-突触核蛋白具有强烈的免疫反应性。我们以前已经表明，从路易体痴呆和多系统萎缩脑中分离的细丝被标记在一个特征性的方式由一些α-突触核蛋白抗体。在这里，我们已经从原发性帕金森病患者的黑质中提取了细丝。针对α-突触核蛋白的羧基末端区域的抗体沿着其整个长度标记分离的细丝。相比之下，针对α-突触核蛋白的氨基末端区域的抗体仅标记一个细丝末端。这些特征与从路易体痴呆和多系统萎缩患者脑中提取的纤维相同。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
The defining neuropathological deposits of Parkinson's disease, dementia with Lewy bodies and multiple system atrophy are strongly immunoreactive for alpha-synuclein. We have shown previously that isolated filaments from dementia with Lewy bodies and multiple system atrophy brains are labelled in a characteristic fashion by a number of alpha-synuclein antibodies. Here we have extracted filaments from substantia nigra of patients with idiopathic Parkinson's disease. Antibodies directed against the carboxy-terminal region of alpha-synuclein labelled isolated filaments along their entire lengths. By contrast, an antibody directed against the amino-terminal region of alpha-synuclein only labelled one filament end. These characteristics were identical to those of fi laments extracted from brains of patients with dementia with Lewy bodies and multiple system atrophy. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.