INDUCTION OF MACROPHAGE PRODUCTION AND PROLIFERATION BY A PURIFIED COLONY STIMULATING FACTOR
INDUCTION OF MACROPHAGE PRODUCTION AND PROLIFERATION BY A PURIFIED COLONY STIMULATING FACTOR
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DOI：
10.1038/274168a0
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发表时间：
1978-01-01
期刊：
影响因子：
64.8
通讯作者：
LIN, HS
中科院分区：
文献类型：
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作者：
STANLEY, ER;CHEN, DM;LIN, HS
集落刺激因子（CSF）在体外刺激分离的造血祖细胞分化并形成粒细胞和/或巨噬细胞集落1，2。CSF似乎至少有两种不同的亚类，刺激巨噬细胞集落形成的亚类和刺激粒细胞集落形成的亚类。从小鼠L细胞条件培养基中纯化的CSF是一种分子量为70，000的糖蛋白，包含两个二硫键键合的35，000 MW亚基3。在低至10− 12 M的浓度下，它刺激小鼠骨髓细胞形成巨噬细胞集落。它与人尿CSF具有相似的理化性质，并具有共同的抗原决定簇3 -6。这种类型的CSF已被证明与Virolainen和Defendi的巨噬细胞生长因子6具有相同性，7刺激腹膜渗出液巨噬细胞增殖。在Lin和Stewart 8的琼脂培养系统中，纯化的CSF也具有刺激腹腔渗出细胞形成巨噬细胞集落的活性6。我们现在报告，这种相同类型的CSF是负责从几个造血和非造血组织的单核吞噬细胞的增殖的分子。
COLONY STIMULATING FACTORS (CSFs) stimulate isolated haemopoietic progenitor cells to differentiate and form granulocyte and/or macrophage coloniesin vitro1,2. There seem to be at least two distinct subclasses of CSF, those stimulating macrophage colony formation and those stimulating granulocytic colony formation. Purified CSF from mouse L cell-conditioned medium is a 70,000 molecular weight glycoprotein, comprising two disulphide-bonded 35,000 MW subunits3. At concentrations as low as 10−12M it stimulates macrophage colony formation by murine bone marrow cells. It has similar physicochemical properties to, and shared antigenic determinants with, the CSF from human urine3–6. This type of CSF has been shown6to share identity with the macrophage growth factor of Virolainen and Defendi7that stimulates proliferation of peritoneal exudate macrophages. The purified CSF was also active6in stimulating macrophage colony formation by peritoneal exudate cells in the agar culture system of Lin and Stewart8. We now report that this same kind of CSF is the molecule responsible for the proliferation of mononuclear phagocytes from several haemopoietic and nonhaemopoietic tissues.