Urine proteomics analysis of dairy cows with fatty liver.
Urine proteomics analysis of dairy cows with fatty liver.
复制标题
DOI：
--
复制
发表时间：
2016
影响因子：
2.3
通讯作者：
C. Xu;Y. Sun;W. Yang;L. Wu;H. Y. Zhang;C. Xia
中科院分区：
文献类型：
--
作者：
C. Xu;Y. Sun;W. Yang;L. Wu;H. Y. Zhang;C. Xia
收稿日期: 修订日期: 已接受日期: 在线发布日期: 2015年1月14日 2015年7月24日 2015年8月15日 2015年10月7日 本研究的目的是筛选和鉴定脂肪肝病（FLD）奶牛尿液样本中的差异蛋白，为FLD提供新的生物标志物。在这项试验中，收集了二十份尿液样本并分为两组。用i-TRAQ试剂标记后，利用二维色谱分离尿样中的差异蛋白，然后通过串联质谱法进行鉴定。结果显示，筛选出110个差异蛋白：FLD奶牛尿液中50个上调，60个下调。其中，玻璃连接蛋白（VTN）、中性粒细胞明胶酶相关脂粘蛋白（LCN2）、凝血酶原（F2）和簇蛋白（CLU）是通过基因功能、信号通路和基因网络分析确定的关键调控因子。因此，相信这4个关键调控因子可能成为FLD的重要生物标志物，为奶牛FLD的临床病理学研究提供新的见解。 ©2015 PVJ。版权所有
Received: Revised: Accepted: Online available: January 14, 2015 July 24, 2015 August 15, 2015 October 07, 2015 The objective of this study was to screen and identify differential proteins in the urine samples from cows with fatty liver disease (FLD) in order to provide new biomarkers for FLD. In this trial, twenty urine specimen were collected and assigned into two groups. After labelling with an i-TRAQ reagent, differential proteins in the urine samples were separated using two dimensional chromatography, and then identified by tandem mass spectrometry. The results showed that 110 differential proteins were screened: 50 were upregulated and 60 were down-regulated in the urine of cows with FLD. Among of these proteins, vitronection (VTN), neutrophil gelatinase-associated lipocatin (LCN2), prothrombin (F2), and clusterin (CLU) were the key regulatory factors determined by gene function, signaling pathway, and gene networks analysis. So, it is believed that the 4 key regulatory factors may become important biomarkers of FLD, which will provide new clinicopathologic insights into FLD in dairy cows. ©2015 PVJ. All rights reserved