The Fecal Microbiota Profile and Bronchiolitis in Infants
The Fecal Microbiota Profile and Bronchiolitis in Infants
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DOI：
10.1542/peds.2016-0218
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发表时间：
2016-07-01
期刊：
影响因子：
8
通讯作者：
Camargo, Carlos A., Jr.
中科院分区：
文献类型：
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作者：
Hasegawa, Kohei;Linnemann, Rachel W.;Camargo, Carlos A., Jr.
背景：肠道菌群是一种潜在的可改变因素，对婴儿毛细支气管炎的相关性知之甚少。我们的目的是确定粪便微生物群与婴儿毛细支气管炎的关系。方法：我们进行了一项病例对照研究。作为多中心前瞻性研究的一部分，我们收集了40例毛细支气管炎住院婴儿的粪便样本。我们同时招募了115名年龄匹配的健康对照。通过应用16 S rRNA基因测序和无偏聚类方法，这155个粪便样本，我们确定了微生物群的配置文件，并确定了微生物群配置文件与毛细支气管炎的可能性的关联。结果：总体而言，中位年龄为3个月，55%是男性，54%是非西班牙裔白色。粪便微生物群的无偏聚类确定了4种不同的谱：土方肠杆菌属优势谱（30%）、双歧杆菌属优势谱（21%）、肠杆菌/韦荣球菌属优势谱（22%）和拟杆菌属优势谱（28%）。毛细支气管炎的比例在肠杆菌属/韦荣氏球菌属占优势的婴儿中最低（15%），在类杆菌属占优势的婴儿中最高（44%），相应的比值比为4.59（95%置信区间，1.58-15.5; P = 0.008）。在多变量模型中，类杆菌属占优势的特征与毛细支气管炎的可能性更大之间的显著相关性持续存在（与肠杆菌属/韦荣球菌属占优势的特征相比，比值比为4.24; 95%置信区间为1.56-12.0; P = 0.005）。与此相反，在婴儿的可能性毛细支气管炎的土方肠杆菌属占主导地位或双歧杆菌属占主导地位的配置文件相比，没有显着不同的肠杆菌/韦荣球菌属占主导地位的profile.CONCLUSIONS：在这个病例对照研究中，我们确定了4个不同的粪便微生物群配置文件的婴儿。类杆菌优势型与毛细支气管炎的可能性较高相关。
BACKGROUND: Little is known about the association of gut microbiota, a potentially modifiable factor, with bronchiolitis in infants. We aimed to determine the association of fecal microbiota with bronchiolitis in infants.METHODS: We conducted a case-control study. As a part of multicenter prospective study, we collected stool samples from 40 infants hospitalized with bronchiolitis. We concurrently enrolled 115 age-matched healthy controls. By applying 16S rRNA gene sequencing and an unbiased clustering approach to these 155 fecal samples, we identified microbiota profiles and determined the association of microbiota profiles with likelihood of bronchiolitis.RESULTS: Overall, the median age was 3 months, 55% were male, and 54% were non-Hispanic white. Unbiased clustering of fecal microbiota identified 4 distinct profiles: Escherichiadominant profile (30%), Bifidobacterium-dominant profile (21%), Enterobacter/Veillonelladominant profile (22%), and Bacteroides-dominant profile (28%). The proportion of bronchiolitis was lowest in infants with the Enterobacter/Veillonella-dominant profile (15%) and highest in the Bacteroides-dominant profile (44%), corresponding to an odds ratio of 4.59 (95% confidence interval, 1.58-15.5; P =.008). In the multivariable model, the significant association between the Bacteroides-dominant profile and a greater likelihood of bronchiolitis persisted (odds ratio for comparison with the Enterobacter/Veillonella-dominant profile, 4.24; 95% confidence interval, 1.56-12.0; P =.005). In contrast, the likelihood of bronchiolitis in infants with the Escherichia-dominant or Bifidobacterium-dominant profile was not significantly different compared with those with the Enterobacter/Veillonella-dominant profile.CONCLUSIONS: In this case-control study, we identified 4 distinct fecal microbiota profiles in infants. The Bacteroides-dominant profile was associated with a higher likelihood of bronchiolitis.