Image-based high-throughput quantification of cellular fat accumulation
Image-based high-throughput quantification of cellular fat accumulation
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DOI：
10.1177/1087057107306502
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发表时间：
2007-10-01
影响因子：
--
通讯作者：
O'Carroll, Simon
中科院分区：
文献类型：
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作者：
Dragunow, Mike;Cameron, Rachel;O'Carroll, Simon
A number of biochemical methods are available for measuring fat accumulation in cell culture. The authors report a simple image-based method for measuring fat accumulation in adipocytes using a combination of high-throughput brightfield microscopy and image analysis, which was validated biochemically using Oil-Red-O. The quickest and most accurate method of analysis was one based on thresholding brightfield images and determining the area of fat droplets per image. Thus, the authors have developed a simple high-throughput, label-free method for measuring fat accumulation that is applicable to any cell or tissue type where fat droplets are visible under light microscopy.
