Glycoprofiling of bifidobacterial consumption of human milk oligosaccharides demonstrates strain specific, preferential consumption of small chain glycans secreted in early human lactation
Glycoprofiling of bifidobacterial consumption of human milk oligosaccharides demonstrates strain specific, preferential consumption of small chain glycans secreted in early human lactation
复制标题
DOI：
10.1021/jf0710480
复制
发表时间：
2007-10-31
影响因子：
6.1
通讯作者：
German, J. Bruce
中科院分区：
文献类型：
--
作者：
Locascio, Riccardo G.;Ninonuevo, Milady R.;German, J. Bruce
人类母乳支持婴儿肠道微生物群发育的分子基础尚不清楚。在乳糖和脂类之后，人乳低聚糖(HMOS)在数量上是母乳中第三大和最多样化的成分。在这项工作中，利用傅立叶变换离子回旋共振质谱仪对双歧杆菌消耗HMO的糖谱分析表明，从婴儿肠道分离的长双歧杆菌ATCC 15697优先消耗小质量低聚糖，占可用HMO总量的63.9%。利用高效液相色谱-芯片飞行时间质谱仪，在哺乳开始时和哺乳第一个月期间一直在母乳中检测到这些HMO。进一步的鉴定表明，菌株ATCC 15697同时具有岩藻糖苷酶和唾液酸酶活性，这是其他受试菌株所没有的。这项工作提供的证据表明，这些小质量的HMO可以被选定的双歧杆菌菌株选择性代谢，并代表了一类潜在的新的生物活性分子，作为益生元，促进母乳喂养新生儿的保护性肠道定植。
The molecular basis by which human breast milk supports the development of a protective intestinal microbiome in infants is unknown. After lactose and lipids, human milk oligosaccharides (HMOs) are quantitatively the third largest and most diverse component of breast milk. In this work, glycomic profiling of HMO consumption by bifidobacteria using Fourier transform ion cyclotron resonance mass spectrometry reveals that one species, Bifidobacterium longum biovar infantis ATCC 15697, an isolate from the infant gut, preferentially consumes small mass oligosaccharides, representing 63.9% of the total HMOs available. These HMOs were detected in human breast milk at the onset and constantly through the first month of lactation by use of high performance liquid chromatography-chip time-of-flight mass spectrometry. Further characterization revealed that strain ATCC 15697 possesses both fucosidase and sialidase activities not present in the other tested strains. This work provides evidence that these small mass HMOs are selectively metabolized by select bifidobacterial strains and represent a potential new class of bioactive molecules functioning as prebiotics to facilitate a protective gut colonization in breast-fed newborns.