Prospects of Deep Raman Spectroscopy for Noninvasive Detection of Conjugated Surface Enhanced Resonance Raman Scattering Nanoparticles Buried within 25 mm of Mammalian Tissue
Prospects of Deep Raman Spectroscopy for Noninvasive Detection of Conjugated Surface Enhanced Resonance Raman Scattering Nanoparticles Buried within 25 mm of Mammalian Tissue
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DOI：
10.1021/ac100039c
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发表时间：
2010-05-15
影响因子：
7.4
通讯作者：
Matousek, Pavel
中科院分区：
文献类型：
--
作者：
Stone, Nicholas;Faulds, Karen;Matousek, Pavel
这封信讨论了潜在的深拉曼光谱，表面增强空间偏移拉曼光谱（SESORS及其变种），非侵入性检测小，深埋病变使用表面增强共振拉曼散射（SERRS）活性纳米粒子。在透射拉曼几何中进行了这一概念的实验演示。这种方法开辟了在体内，非侵入性的，具体的检测与疾病相关的分子变化的前景高达几厘米的深度，代表显着改善传统检测的拉曼信号的2个数量级。疾病特异性信号可以使用与靶分子缀合的独特标记的纳米颗粒来实现，例如，用于产生SERRS信号的抗体。这提供了比正常生物拉曼信号大许多数量级的分子特异性信号，并且可以容易地多路复用。迄今为止，还没有研究证明与表面增强技术耦合的深拉曼光谱用于检测组织中深度大于5.5 mm的低浓度感兴趣分子的可行性。这样的突破将在医学诊断中开辟许多新的应用。在这里，我们建议通过将SERRS（作为疾病特异性变化的探针）与深度拉曼光谱技术相结合来促进这种能力。这允许通过15和25 mm厚之间的组织以临床相关浓度对来自缀合的SERRS纳米颗粒的拉曼特征进行非侵入性测量。
This letter discusses the potential of deep Raman spectroscopy, surface enhanced spatially offset Raman spectroscopy (SESORS and its variants), for noninvasively detecting small, deeply buried lesions using surface enhanced resonance Raman scattering (SERRS) active nanoparticles. An experimental demonstration of this concept is performed in transmission Raman geometry. This method opens prospects for in vivo, noninvasive, specific detection of molecular changes associated with disease up to depths of several centimeters representing significant improvement over traditionally detected Raman signals by 2 orders of magnitude. The disease specific signals can be achieved using uniquely tagged nanoparticles conjugated to target molecules, e.g., antibodies for production of the SERRS signal. This provides the molecular specific signal which is many orders of magnitude greater than normal biological Raman signals and can be easily multiplexed. To date, there have been no studies demonstrating the viability of deep Raman spectroscopy coupled to surface enhanced techniques for detecting low concentrations of molecules of interest at depths of greater than 5.5 mm in tissue. Such a breakthrough would open a host of new applications in medical diagnoses. Here we propose to facilitate such capability by combining SERRS (as a probe for disease specific changes) with deep Raman spectroscopy techniques. This permits noninvasive measurement of Raman signatures from conjugated SERRS nanoparticles at clinically relevant concentrations through tissues of between 15 and 25 mm thick.