Isolation and characterization of a new strain of Methanothermobacter marburgensis DX01 from hot springs in China
Isolation and characterization of a new strain of Methanothermobacter marburgensis DX01 from hot springs in China
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DOI：
10.1016/j.anaerobe.2009.04.001
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发表时间：
2010-02-01
期刊：
影响因子：
2.3
通讯作者：
Lu, Zhen-Mei
中科院分区：
文献类型：
--
作者：
Ding, Xia;Yang, Wei-Jun;Lu, Zhen-Mei
菌株DX01是从中国某温泉中分离得到的嗜热产甲烷菌。菌株DX01仅在H(2)/CO(2)条件下生长。DNA G + C含量为52 mol%，最佳生长温度为65℃，细胞颗粒呈砖红色。通过16S rRNA序列、甲基辅酶M还原酶1、γ亚基蛋白序列分析，确定DX01菌株与马布尔根产甲烷热杆菌亲缘关系密切。此外，产热自养甲烷杆菌δ HT与菌株DX01在生化组成和蛋白表达谱上存在明显差异。基于以上分析，我们提出菌株DX01是马布氏分枝杆菌热自养菌种中的一个新菌株，即马布氏分枝杆菌DX01。马布氏分枝杆菌DX01菌株的分离和鉴定扩大了已知热甲烷菌属的范围。爱思唯尔有限公司2009年版权所有版权所有。
Strain DX01, a thermophilic methanogen, was isolated from a hot spring in China. Strain DX01 grew only on H(2)/CO(2). The DNA G + C content is 52 mol% and optimal growth temperature is 65 degrees C. The cell pellet is brick red. By analyzing 16S rRNA sequence, methyl-coenzyme M reductase 1, gamma subunit protein sequences, we determined the DX01 strain to be closely related to the species of Methanothermobacter marburgensis. In addition, Methanothermobacter thermautotrophicus delta HT and strain DX01 had clear differences in their biochemical composition and protein expression profiles. Based on the above analysis, we propose that strain DX01 is a novel strain within thermloautotrophicus the species of M. marburgensis, namely M. marburgensis DX01. The isolation and characterization of the new M. marburgensis DX01 strain expands the known range of the Methanothermobacter genus. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.