Biomimetic Graphene-Based 3D Scaffold for Long-Term Cell Culture and Real-Time Electrochemical Monitoring
Biomimetic Graphene-Based 3D Scaffold for Long-Term Cell Culture and Real-Time Electrochemical Monitoring
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DOI：
10.1021/acs.analchem.7b03324
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发表时间：
2018-01-16
影响因子：
7.4
通讯作者：
Huang, Wei-Hua
中科院分区：
文献类型：
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作者：
Hu, Xue-Bo;Liu, Yan-Ling;Huang, Wei-Hua
目前在细胞电化学监测方面取得的成果通常是在二维（2D）衬底上获得的，这种衬底无法模拟细胞环境并准确地再现细胞内部的功能。三维（3D）组织因此，同时具有细胞培养和电化学传感功能的3D支架有望成为一种对细胞在体内三维微环境下进行实时监测的平台。然而，构建这样一个多功能支架平台是一个特别具有挑战性的问题。在此，我们开发了一种3-氨基苯基硼酸（APBA）功能化石墨烯泡沫（GF）网络，该网络将APBA的仿生性能与GF的力学和电化学性能结合在一起。因此，GF网络可以作为长时间高活力培养细胞的3D支架，同时作为高灵敏度电化学传感的电极。这样就可以实时监测3D支架上培养的细胞释放的气态信使H2S。这项工作代表了具有电化学特性的3D细胞培养支架的制造取得了相当大的进展，从而促进了未来生理学相关过程的研究。
Current achievements on electrochemical monitoring of cells are often gained on two-dimensional (2D) substrates, which fail in mimicking the cellular environments and accurately reproducing the cellular functions within. a three-dimensional (3D) tissue. In this regard, 3D scaffold concurrently integrated with the function of cell culture and electrochemical sensing is conceivably a promising platform to monitor cells in real time under their in vivo-like 3D microenvironments. However, it is particularly challenging to construct such a multifunctional scaffold platform. Herein, we developed a 3-aminophenylboronic acid (APBA) functionalized graphene foam (GF) network, which combines the biomimetic property of APBA with the mechanical and electrochemical properties of GF. Hence, the GF network can serve as a 3D scaffold to culture cells for a long period with high viability and simultaneously as an electrode for highly sensitive electrochemical sensing. This allows monitoring of gaseous messengers H2S released from the cells cultured on the 3D scaffold in real time. This work represents considerable progress in fabricating 3D cell culture scaffold with electrochemical properties, thereby facilitating future studies of physiologically relevant processes.