Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge
Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge
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DOI：
10.1089/ten.2005.11.1346
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发表时间：
2005-09-01
影响因子：
--
通讯作者：
Kameyama, Y
中科院分区：
文献类型：
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作者：
Fujita, M;Kinoshita, Y;Kameyama, Y
采用胶原海绵、聚羟基乙酸（PGA）-胶原海绵和PGA-胶原（UV）海绵3种支架材料，研究地塞米松（Dex）和碱性成纤维细胞生长因子（bFGF）对大鼠骨髓基质细胞（RBMSCs）增殖和分化的影响。将RBMSCs接种于海绵中，并在原代培养基、含Dex的原代培养基和含bFGF和Dex的原代培养基中培养。培养三周后，我们检查了碱性磷酸酶（ALP）的活性和细胞数量的海绵，并进行了宏观，光学显微镜和扫描电子显微镜（SEM）的观察。胶原蛋白海绵收缩相当大，但PGA-胶原蛋白和PGA-胶原蛋白（UV）海绵保持其原始形状的大部分。同时添加bFGF和Dex的PGA-胶原（UV）海绵的ALP活性和细胞数量最高，其次是PGA-胶原海绵。胶原海绵仅在表面显示细胞增殖，而其他两种海绵在内部显示细胞增殖。SEM显示，在bFGF和Dex存在下，细胞在PGA-胶原（UV）海绵上的附着最好，其次是PGA-胶原海绵。总之，PGA-胶原（UV）和PGA-胶原海绵被证明是更有用的支架骨再生时，结合bFGF和地塞米松。
We studied the effects of dexamethasone ( Dex) and basic fibroblast growth factor ( bFGF) on proliferation and differentiation of rat bone marrow stromal cells ( RBMSCs), using three scaffolds: collagen sponge, poly( glycolic acid) ( PGA)-collagen sponge, and PGA-collagen ( UV) sponge. RBMSCs were seeded into the sponges, and cultured in primary medium, primary medium with Dex, and primary medium with bFGF and Dex. Three weeks after cultivation, we examined alkaline phosphatase ( ALP) activity and cell number in the sponges, and also performed macroscopic, light microscopic, and scanning electron microscopic ( SEM) observations. Collagen sponge shrank considerably, but PGA-collagen and PGA-collagen ( UV) sponges maintained most of their original shape. PGA-collagen ( UV) sponge supplemented with bFGF and Dex together had the highest ALP activity and cell number, followed by PGA-collagen sponge. Although collagen sponge showed cell proliferation only on the surface, the other two sponges showed cell proliferation in the interior. SEM showed the best cell attachment to PGA-collagen ( UV) sponge in the presence of bFGF and Dex, followed by PGA-collagen sponge. In conclusion, PGA-collagen ( UV) and PGA-collagen sponges proved to be much more useful as scaffolding for bone regeneration when combined with bFGF and Dex.