Chages in Carbohydrate and Ribulose Bisphosphate Carboxylase-Oxygenase Contents in Peach Leaves after Applications of Different Amounts of Nitrogen Fertilizer
Chages in Carbohydrate and Ribulose Bisphosphate Carboxylase-Oxygenase Contents in Peach Leaves after Applications of Different Amounts of Nitrogen Fertilizer
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施用不同量氮肥后桃叶碳水化合物和二磷酸核酮糖羧化酶加氧酶含量的变化
DOI：
10.2503/jjshs.66.505
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发表时间：
1997
影响因子：
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通讯作者：
M. Nishimura
中科院分区：
文献类型：
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作者：
N. Nii;K. Yamaguchi;M. Nishimura
研究了不同施氮量对桃树幼树非结构碳水化合物和二磷酸核酮糖羧化酶（RuBisCO）积累及部分性状的影响。施氮量为0和1.5g /盆（N-0和N-1.5）的树木叶片氮含量低于施氮量为9.0g/盆（N-9）的树木。在干重（DW）的基础上，N-0树和N-9树的叶片取样率分别为1.8%和4.0%。单位叶面积叶绿素含量随施氮量的增加而增加。以干重为基础的淀粉含量与施氮量成反比，而每单位干重的总可溶性糖则成正比。单位叶鲜重山梨醇和蔗糖含量以N-0树最低。随着施氮量的增加，sds -聚丙烯酰胺凝胶电泳后的免疫印迹和光镜下切片的免疫染色检测的RuBisCO含量增加。
The effects of applying different amounts of nitrogen on the accumulation of nonstructural carbohydrate and ribulose bisphosphate carboxylase-oxygenase (RuBisCO) and on some characteristics of peach leaves were determined on young trees. The leaf nitrogen contents of trees supplied 0 and 1.5g N/pot (N-0 and N-1.5, respectively) were lower than were those administered 9.0g/pot (N-9). On a dry weight (DW) basis, leaves sampled from N-0 trees and N-9 trees were 1.8% and 4.0%, respectively. The chlorophyll content per unit leaf area increased with increased nitrogen applied. The starch content on a dry weight basis was inversely proportional to the amount of nitrogen administered, whereas total soluble sugar per unit dry weight was proportional. The sorbitol and sucrose contents per unit leaf fresh weight were the lowest in leaves from N-0 trees. The RuBisCO content detected by immunoblotting after SDS-polyacrylamide gel electrophoresis as well as by immunostaining of sections under the light microscope increased with the increase in the amounts of nitrogen applied.
Sawada, S.、Kawamura, H. Hayakawa, T. 和 Kasai, M.：“单根大豆植物根部低温处理对光合代谢的调节”植物
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