Neurons recorded from pediatric epilepsy surgery patients with cortical dysplasia
Neurons recorded from pediatric epilepsy surgery patients with cortical dysplasia
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DOI：
10.1111/j.1528-1157.2000.tb01575.x
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发表时间：
2000-01-01
期刊：
影响因子：
5.6
通讯作者：
Levine, MS
中科院分区：
文献类型：
--
作者：
Mathern, GW;Cepeda, C;Levine, MS
目的：皮质发育不良（CD）是早发性儿童癫痫患者常见的病理底物。在乳糜泻组织中，对细胞高兴奋性的机制知之甚少。在这项研究中，我们报告了儿童CD患者正常和畸形细胞的初始电生理和形态学观察。方法：对15例CD患者（年龄0.3 ~ 14岁）的神经影像学和皮质电图进行“最”和“最”致痫区采样。全细胞电压钳记录结合红外视频显微镜，将同一患者的异常细胞（巨细胞神经元、分叉树突细胞、失向锥体细胞等）与正常神经元进行比较。细胞充满生物细胞素，并对相邻组织块进行神经元和胶质标记染色。结果：161个细胞中约15%外观正常。在应用嗜离子受体激动剂后，异常细胞表现出从细胞固有的高兴奋性到低敏感性的电生理异常。其他发现包括兴奋性突触后电流增加和γ -氨基丁酸逆转电位的改变。结论：在儿童CD组织中，这些初步结果表明，与正常神经元相比，异常细胞在电生理指标上表现出异常。异常从高兴奋性到低兴奋性不等。随着更多患者资料的分析，将会有更详细的结果和结论。
Purpose: Cortical dysplasia (CD) is a common pathological substrate in patients with early-onset childhood epilepsy. In CD tissue, little is known about the mechanisms responsible for cellular hyperexcitability. In this study, we report initial electrophysiological and morphological observations from normal and dysmorphic cells in pediatric CD patients.Methods: Neocortical "most" and "least" epileptogenic areas were sampled based on neuroimaging and electrocorticography from 15 CD patients (ages 0.3 to 14 years). Whole-cell voltage clamp recordings combined with infrared videomicroscopy sampled abnormal cells (cytomegalic neurons, cells with bifurcated dendrites, disoriented pyramidal cells, etc.) compared with normal-appearing neurons from the same patient. Cells were filled with biocytin, and adjacent tissue blocks were stained for neuronal and glial markers.Results: About 15% of the 161 recorded cells were al,normal in appearance. Abnormal cells showed electrophysiological irregularities ranging from intrinsic cellular hyperexcitability to hyposensitivity after application of ionotropic receptor agonists. Other findings included increased excitatory postsynaptic currents and alterations in gamma-aminobutyric acid reversal potentials.Conclusions: In pediatric CD tissue, these preliminary results indicate that abnormal-appearing cells showed abnormalities in electrophysiological measures compared with normal appearing neurons. The abnormalities varied from hyperexcitability to hypoexcitability. More detailed results and conclusions will be forthcoming as additional patient material is analyzed.