Plant type differently promote the arbuscular mycorrhizal fungi biodiversity in the rhizosphere after revegetation of a degraded, semiarid land
Plant type differently promote the arbuscular mycorrhizal fungi biodiversity in the rhizosphere after revegetation of a degraded, semiarid land
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DOI：
10.1016/j.soilbio.2010.09.029
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发表时间：
2011-01-01
影响因子：
9.7
通讯作者：
Roldan, A.
中科院分区：
文献类型：
--
作者：
Alguacil, M. M.;Torres, M. P.;Roldan, A.
有人建议，丛枝菌根真菌（AMF）的多样性和它们与不同的植物物种的关联是至关重要的，在早期阶段的植被重建程序，因为AMF根定殖起着重要的作用，提高植物的建立和生长。我们进行了一项研究，我们分析了在西班牙东南部的地中海半干旱退化地区的脆弱麻黄，Rhamnus lycioides，阿月浑子lentiscus和Retama sphaerocarpa重新植被14个月后，在根中的AM真菌群落组成，以验证是否不同的植物物种种植后，可以共同促进AM真菌在其根际的多样性。我们分析了一部分约795碱基对的小亚基核糖体DNA通过巢式PCR，克隆，测序和系统发育分析。鉴定了八种真菌序列类型，分别属于球囊霉属A组和B组以及副球囊霉属。不同植物种类AM真菌群落组成不同。因此，R. lycioides的4种序列型最多，而E. fragilis只有两种类型可以被表征为该植物物种特有的。P.lentiscus和R. sphaerocarpa具有3种层序类型，其中2种为共有层序。所有的AMF序列类型都在天然土壤中发现。这些结果表明，恢复退化土地的一个有效途径是增加植物物种的数量，这将增加土壤中AMF的多样性，从而增加地下的积极相互作用。(C)2010爱思唯尔有限公司版权所有。
It is suggested that the diversity of arbuscular mycorrhizal fungi (AMF) and their association with distinct plants species are crucial in the early stages of revegetation procedures since the AMF roots colonisation plays an important role improving plant establishment and growth. We carried out a study where we analyse the AMF community composition in the roots of Ephedra fragilis, Rhamnus lycioides, Pistacia lentiscus and Retama sphaerocarpa fourteen months after revegetation in a Mediterranean semiarid degraded area of southeast Spain in order to verify whether different plant species can variably promote the diversity of AM fungi in their rhizospheres after planted. We analysed a portion of approximately 795 bases pairs of the small-subunit ribosomal DNA by means of nested PCR, cloning, sequencing and phylogenetic analyses. Eight fungal sequence types belonging to Glomus group A and B and to the genus Paraglomus were identified. The different plant species had different AM fungal community composition. Thus, R. lycioides harboured the highest number of four fungal sequence types while from E. fragilis only two types could be characterized that were specific for this plant species. P. lentiscus and R. sphaerocarpa harboured each one three sequence types and two of them were shared. All AMF sequence types were found in the natural soil. These results show that one effective way of restoring degraded lands is to increase the number of plant species used, which would increase the AMF diversity in the soil and thus the below-ground, positive interactions. (C) 2010 Elsevier Ltd. All rights reserved.