Prolonged depolarization of rat cerebral synaptosomes leads to an increase in vesicular glutamate content.
Prolonged depolarization of rat cerebral synaptosomes leads to an increase in vesicular glutamate content.
复制标题
大鼠大脑突触体的长期去极化导致囊泡谷氨酸含量增加。
DOI：
10.1016/s0304-3940(02)00105-2
复制
发表时间：
2002
影响因子：
2.5
通讯作者：
Ueda,Tetsufumi
中科院分区：
文献类型：
--
作者：
Bole,DavidG;Hirata,Koji;Ueda,Tetsufumi
Glutamate accumulation into synaptic vesicles is a vital step in glutamate synaptic transmission. In this study, we have explored the possibility that vesicular glutamate storage may be subject to some regulation. Synaptosomes were depolarized and subjected to [3H] glutamate under non-depolarizing conditions, and vesicular [3H] glutamate content was determined by a filter-based assay. We present evidence here that prolonged depolarization of synaptosomes leads to an increase in vesicular glutamate content. Induction of this enhanced state is time- and temperature-dependent. The enhanced state has two components, one readily reversible and the other long-lasting. The up-regulation of glutamate storage capacity could lead to an increase in quantal size and play a role in modulation of glutamate transmission efficiency.
