Immunolocalisation studies of matrix metalloproteinases-1,-2 and-3 in human melanoma
Immunolocalisation studies of matrix metalloproteinases-1,-2 and-3 in human melanoma
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DOI：
10.1007/s004280050443
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发表时间：
1999-12-01
期刊：
影响因子：
--
通讯作者：
Woolley, DE
中科院分区：
文献类型：
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作者：
Walker, RA;Woolley, DE
基质金属蛋白酶（MMPs）被认为在结缔组织降解中起重要作用，并与肿瘤侵袭和转移扩散的机制有关。我们采用免疫组织化学方法检测并比较了18例恶性黑色素瘤（10例浅表扩散型黑色素瘤和8例结节性黑色素瘤）标本中胶原酶-1 （MMP-1）、明胶酶A （MMP-2）和基质溶素-1 （MMP-3）的组织分布。mmp -1， -2和-3在黑色素瘤和宿主组织细胞中被证实，特别是在一些谣言的周围，但通常仅限于总黑色素瘤细胞的10%以下。远离肿瘤的“正常”皮肤组织中不存在MMPs。MMP-2定位于离散的细胞群，在表皮肿瘤界面尤其明显，而MMP-3主要局限于黑色素瘤的深层边缘。表面扩散或结节性黑色素瘤均未见MMP表达规律。MMPs的可变分布表明，酶的表达受局部微环境调节，可能与肿瘤边缘观察到的基质成分和细胞异质性有关。这些原位观察增加了特异性MMPs有助于肿瘤侵袭机制的概念的权重。
The matrix metalloproteinases (MMPs) are considered to have an important role in connective tissue degradation and have been implicated in the mechanisms of tumour invasion and metastatic spread. We have used immunohistochemistry to examine and compare the tissue distributions of collagenase-1 (MMP-1), gelatinase A (MMP-2) and stromelysin-1 (MMP-3) in 18 specimens of malignant melanoma, viz. 10 superficial spreading and 8 nodular melanomas. MMPs-1, -2 and -3 were demonstrated within melanoma and host tissue cells, especially at the periphery of some rumours, but were usually restricted to less than 10% of total melanoma cells. The MMPs were absent from 'normal' skin tissue distant from the tumour. MMP-2 was localised to discrete groups of cells and was especially evident at the epidermal:tumour interface, whereas MMP-3 was mainly confined to the deeper margins of melanoma. No regular pattern of MMP expression was observed for either the superficial spreading or the nodular melanomas. The variable distributions of the MMPs suggested that enzyme expression was subject to local microenvironmental regulation, possibly in response to matrix components and the cellular heterogeneity observed at the tumour margins. These in situ observations add weight to the concept that specific MMPs contribute to the mechanisms of tumour invasion.