Duration of viraemia infectious to Culicoides sonorensis in bluetongue virus-infected cattle and sheep
Duration of viraemia infectious to Culicoides sonorensis in bluetongue virus-infected cattle and sheep
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DOI：
10.1016/s0378-1135(02)00106-2
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发表时间：
2002-08-25
影响因子：
3.3
通讯作者：
MacLachlan, NJ
中科院分区：
文献类型：
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作者：
Bonneau, KR;DeMaula, CD;MacLachlan, NJ
通过将实验室饲养的索诺库蠓 (C sonorensis) 直接喂入先前通过昆虫接种感染 BTV 的反刍动物的皮肤上，评估了感染蓝舌病毒 (BTV) 的绵羊和牛的病毒血症持续时间。通过病毒分离和逆转录酶（RT）巢式聚合酶链反应（PCR）测定来确定感染后感染性BTV和BTV核酸分别出现在受感染牛和羊的血液中的时间间隔。感染后 7、21 和 49 天 (p.i.) 还通过病毒分离和 RT-PCR 测定确定了以 BTV 感染的牛和羊为食的媒介昆虫中 BTV 的存在。 BTV 在接种后长达 49 天的时间内从受感染牛的血液中分离出来，而在接种后 11 天后仍未从感染 BTV 的绵羊中分离出 BTV。相比之下，在感染反刍动物的血液中检测到 BTV 核酸，持续时间为 111-222 天。感染 C. sonorensis 的病毒血症的最长持续时间为 21 天。牛和羊均受到感染，除了注射后 21 天的一只羊外，只有通过病毒分离确定其血液中含有 BTV 的反刍动物才能在口服喂养后感染 C. sonorensis。这项研究和之前的研究数据表明，病毒血症在 BTV 感染的反刍动物中是暂时的，RT 巢式 PCR 检测为牛和羊是否存在 BTV 筛查提供了非常灵敏和保守的测试。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The duration of viraemia infectious to Culicoides sonorensis (C sonorensis) was evaluated in bluetongue virus (BTV)-infected sheep and cattle by feeding laboratory-reared C sonorensis directly on the skin of ruminants that previously were infected with BTV by insect inoculation. The intervals after infection when infectious BTV and BTV nucleic acids were present in the blood of infected cattle and sheep, respectively, were determined by virus isolation and reverse transcriptase (RT)-nested polymerase chain reaction (PCR) assays. The presence of BTV in vector insects that fed on the BTV-infected cattle and sheep at 7, 21, and 49 days post-infection (p.i.) was also determined by virus isolation and RT-PCR assays. BTV was isolated from the blood of infected cattle for up to 49 days p.i., whereas it was not isolated from the BTV-infected sheep after 11 days p.i. In contrast, BTV nucleic acids were detected in the blood of infected ruminants for 111-222 days p.i. The maximal duration of viraemia that was infectious to C. sonorensis was 21 days p.i. of both cattle and sheep and, with the notable exception of one sheep at 21 days p.i., only ruminants whose blood contained BTV as determined by virus isolation were able to infect C. sonorensis after oral feeding. Data from this and previous studies indicates that viraemia is transient in BTV-infected ruminants, and that the RT-nested PCR assay provides a very sensitive and conservative test for the screening of cattle and sheep for the presence of BTV. (C) 2002 Elsevier Science B.V. All rights reserved.