Neutrophils secrete MIP-1β after adhesion to laminin contained in basement membrane of blood vessels
Neutrophils secrete MIP-1β after adhesion to laminin contained in basement membrane of blood vessels
复制标题
DOI：
10.1111/j.1365-2141.2004.05242.x
复制
发表时间：
2004-12-01
影响因子：
6.5
通讯作者：
Kobayashi, M
中科院分区：
文献类型：
--
作者：
Chiba, KJ;Zhao, WL;Kobayashi, M
我们最近证实，粒细胞集落刺激因子（G-CSF）刺激中性粒细胞产生上皮细胞衍生的中性粒细胞吸引剂-78（ENA-78），ENA-78可能促进从血管内空间迁移到炎症组织的中性粒细胞聚集。在这项研究中，我们检查了是否其他趋化因子可以分泌的中性粒细胞，从血管内空间迁移到炎症组织后积累。我们证明，粘附于基底膜和Matrigel（一种人工基底膜模型）中的层粘连蛋白诱导中性粒细胞中的巨噬细胞炎性蛋白-1 β（MIP-1 β），中性粒细胞分泌的MIP-1 β诱导树突状细胞的趋化性。这些发现表明，通过基底膜迁移的中性粒细胞被基底膜刺激分泌MIP-1 β，诱导树突状细胞从血管内空间迁移到炎症组织中。我们认为中性粒细胞在穿越基底膜的过程中通过分泌MIP-1 β介导天然免疫应答和特异性免疫应答。
We have recently demonstrated that granulocyte colony-stimulating factor (G-CSF) stimulated the production of epithelial-cell-derived-neutrophil attractant-78 (ENA-78) by neutrophils and that ENA-78 might promote the accumulation of neutrophils that had migrated from the intravascular space into inflammatory tissues. In this study, we examined whether other chemokines could be secreted by neutrophils that had accumulated after migrating from the intravascular space into the inflammatory tissues. We demonstrated that adhesion to laminin contained in the basement membrane and Matrigel, which is an artificial basement membrane model, induced macrophage inflammatory protein-1beta (MIP-1beta) in neutrophils and that MIP-1beta secreted by neutrophils induced the chemotaxis of dendritic cells. These findings suggest that neutrophils transmigrating through the basement membrane are stimulated to secrete MIP-1beta by the basement membrane, inducing the transmigration of dendritic cells from the intravascular space into inflammatory tissues. We propose that neutrophils intervene between innate immune response and specific immune response by secreting MIP-1beta during the transmigration through the basement membrane.