Transmission of the agent of human granulocytic ehrlichiosis by Ixodes spinipalpis ticks:: Evidence of an enzootic cycle of dual infection with Borrelia burgdorferi in northern Colorado
Transmission of the agent of human granulocytic ehrlichiosis by Ixodes spinipalpis ticks:: Evidence of an enzootic cycle of dual infection with Borrelia burgdorferi in northern Colorado
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DOI：
10.1086/315715
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发表时间：
2000-08-01
影响因子：
6.4
通讯作者：
Maupin, GO
中科院分区：
文献类型：
--
作者：
Zeidner, NS;Burkot, TR;Maupin, GO
先前的工作描述了广义伯氏疏螺旋体（Borrelia burgdorferi sensu lato）（下文称为B.在北方科罗拉多地区，通过鼠间共感染人粒细胞埃立克体病（aoHGE）的调查，在119只鼠的脾脏中，aoHGE的检出率为23.5%。活组织检查结果显示，119只啮齿动物中有78只（65.5%）为B阳性。28只携带aoHGE的动物中有22只（78.5%）同时感染了B。burgdorferi。在25个I中的14个中，用16 SrRNA和p44基因检测aoHGE。在C3 H/HeJ小鼠中检测了棘须藻传递aoHGE的能力。术后第5天检测到aoHGE。本研究证实了B. burgdorferi和aoHGE可通过I. spinipalpis蜱，啮齿类动物，主要是Peromyscus maniculatus和N，墨西哥，居住在北方科罗拉多的山麓丘陵，有很高的合并感染的发病率。
Previous work described an enzootic cycle of Borrelia burgdorferi sensu lato (hereafter referred to as B. burgdorferi) maintained by the rodent Neotama mexicana and the tick Ixodes spinipalpis in northern Colorado, We investigated the incidence of coinfection among rodents with the agent of human granulocytic ehrlichiosis (aoHGE), aoHGE was detected in 23.5% of 119 rodent spleens examined. Biopsy results indicated that 78 (65.5%) of the 119 rodents were positive for B. burgdorferi, whereas 22 (78.5%) of the 28 animals that harbored aoHGE were also infected with B. burgdorferi. In 14 of 25 I. spinipalpis tick pools, aoHGE was detected by amplifying both the 16s rRNA and p44 gene of aoHGE, The ability of I. spinipalpis to transmit aoHGE was examined in C3H/HeJ mice. aoHGE was detected in their blood 5 days after I. spinipalpis infestation, This study confirms that both B. burgdorferi and aoHGE can be transmitted by I. spinipalpis ticks and that there is a high incidence of coinfection in rodents, predominantly Peromyscus maniculatus and N, mexicana, that inhabit the foothills of northern Colorado.