ADENYLATE ENERGY CHARGE IN SACCHAROMYCES-CEREVISIAE DURING STARVATION
ADENYLATE ENERGY CHARGE IN SACCHAROMYCES-CEREVISIAE DURING STARVATION
复制标题
DOI：
10.1128/jb.121.3.975-982.1975
复制
发表时间：
1975-01-01
影响因子：
3.2
通讯作者：
ATKINSON, DE
中科院分区：
文献类型：
--
作者：
BALL, WJ;ATKINSON, DE
面包师的酵母细胞，酿酒酵母，如果在乙醇上有氧生长，或者如果在葡萄糖上有氧生长，并利用积累的乙醇进入固定相，在长期饥饿期间保持腺苷能荷的正常值(0.8到0.9)。相比之下，在葡萄糖上厌氧生长的细胞和在葡萄糖上有氧生长早期的细胞，如果转移到缺乏能源的培养基中，能量电荷会迅速减少。这些结果表明，在饥饿过程中，有功能的线粒体或腺嘌呤核苷酸酶处于平衡状态。酵母细胞在能量电荷值低于0.1的情况下仍然活着，这与以前在大肠杆菌上获得的结果形成了鲜明的对比。在其他方面，酵母菌对饥饿和再喂食的能量电荷反应与以前报道的大肠杆菌相似。
Bakers' yeast cells, Saccharomyces cerevisiae, if grown aerobically on ethanol or if grown aerobically on glucose and allowed to pass into stationary phase, with utilization of accumulated ethanol, maintain a normal value (0.8 to 0.9) of the adenylate energy charge during prolonged starvation. In contrast, cells grown anaerobically on glucose and cells in the early stages of aerobic growth on glucose exhibit a rapid decrease of energy charge if transferred to medium lacking on energy source. These results suggest that functional mitochondria or enzymes of balance of adenine nucleotides during starvation. Yeast cells remain viable at energy charge values below 0.1, in marked contrast to results previously obtained with Escherichia coli. In other respects, the engery charge responses of yeast to starvation and refeeding are generally similar to those previously reported for E. coli.