An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells
An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells
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DOI：
10.1096/fj.10-157032
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发表时间：
2010-10-01
期刊：
影响因子：
4.8
通讯作者：
Pagano, Aldo
中科院分区：
文献类型：
--
作者：
Castelnuovo, Manuele;Massone, Sara;Pagano, Aldo
神经母细胞瘤（NB）是一种儿科癌症，其特征在于肿瘤结节内显著的细胞异质性。在这里，我们证明了聚合酶III转录的非编码（NC）RNA（NDM 29）的合成强烈限制NB的发展，通过促进细胞分化，恶性过程的下降，并显着减少NB细胞群中的肿瘤起始细胞（TIC）部分。值得注意的是，NDM 29的过表达还赋予恶性NB细胞对NB治疗中使用的抗母细胞药物的作用的不可预测的易感性。总之，这些结果表明诱导NDM 29表达作为可能的治疗以增加癌细胞对治疗剂的脆弱性，并且其在NB外植体中的合成的测量作为这种癌症类型的预后因素。Castelnuovo，M.，Massone，S.，塔索河，巴西-地Fiorino，G.，加蒂，M.，Robello，M.，Gatta，E.，伯杰，A.，Strub，K.，Florio，T.，Dieci，G.，坎塞达河Pagano，A.一种促进细胞分化并降低人神经母细胞瘤细胞恶性程度的类RNA。FASEB J.24，4033-4046（2010）。www.fasebj.org
Neuroblastoma (NB) is a pediatric cancer characterized by remarkable cell heterogeneity within the tumor nodules. Here, we demonstrate that the synthesis of a pol III-transcribed noncoding (nc) RNA (NDM29) strongly restricts NB development by promoting cell differentiation, a drop of malignancy processes, and a dramatic reduction of the tumor initiating cell (TIC) fraction in the NB cell population. Notably, the overexpression of NDM29 also confers to malignant NB cells an unpredicted susceptibility to the effects of antiblastic drugs used in NB therapy. Altogether, these results suggest the induction of NDM29 expression as possible treatment to increase cancer cells vulnerability to therapeutics and the measure of its synthesis in NB explants as prognostic factor of this cancer type.-Castelnuovo, M., Massone, S., Tasso, R., Fiorino, G., Gatti, M., Robello, M., Gatta, E., Berger, A., Strub, K., Florio, T., Dieci, G., Cancedda, R., Pagano, A. An Alu-like RNA promotes cell differentiation and reduces malignancy of human neuroblastoma cells. FASEB J. 24, 4033-4046 (2010). www.fasebj.org