Specificity of PCR and Serological Assays in the Detection of Escherichia coli Shiga Toxin Subtypes
Specificity of PCR and Serological Assays in the Detection of Escherichia coli Shiga Toxin Subtypes
复制标题
DOI：
10.1128/aem.00370-11
复制
发表时间：
2011-09-01
影响因子：
4.4
通讯作者：
Monday, Steven R.
中科院分区：
文献类型：
--
作者：
Feng, Peter C. H.;Jinneman, Karen;Monday, Steven R.
对大肠埃希菌中STX或STX亚型的特异性分析表明，我们检测的方法不能检测到STX(1d)和STX(2f)，也有一些检测不到STX(2b)和STX(2g)。所检测的大多数血清学检测不能检测到Stx2c、Stx2e、Stx2f和Stx2g，并且观察到Stx2b的反应性在株与株之间存在一定的差异。
Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.