Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages
Expression of the zebrafish intermediate neurofilament Nestin in the developing nervous system and in neural proliferation zones at postembryonic stages
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DOI：
10.1186/1471-213x-7-89
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发表时间：
2007-07-25
影响因子：
--
通讯作者：
Driever, Wolfgang
中科院分区：
文献类型：
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作者：
Mahler, Julia;Driever, Wolfgang
背景：据报道，在哺乳动物中枢神经系统（CNS）发育过程中，中间丝巢蛋白是干细胞和特定前体细胞群的标记物。巢蛋白表达的前体可以产生神经元和神经胶质。小鼠巢蛋白的表达始于神经外胚层细胞的神经发育，并在祖细胞分化和有丝分裂后显著下调。据报道，在成年斑马鱼（Danio rerio）中，中枢神经系统的所有主要分支都继续活跃的神经发生，然而斑马鱼前体细胞的标记物很少，并且在斑马鱼中没有描述过巢蛋白。结果：我们克隆了一个斑马鱼巢蛋白gDNA片段，以寻找发育和胚胎后脑中前体细胞的标记。系统进化树分析表明，该斑马鱼与其他脊椎动物的巢蛋白序列同源，但与其他中间丝蛋白不同源。我们分析了nestin在原肠期至4日龄幼虫以及胚胎后脑中的表达。我们发现在体细胞发生过程中广泛表达于神经外胚层。在幼虫中，巢蛋白的表达逐渐局限于所有先前描述的发育中和胚胎后中枢神经系统的增殖区。表达巢蛋白的前脑细胞在胚胎发生后期也表达PCNA，这表明它们是增殖前体细胞或神经干细胞。Nestin也在颅神经节、肌肉细胞的中胚层前体和颅间充质组织中表达。结论：我们的数据表明，在斑马鱼中，与哺乳动物一样，介导的神经丝巢蛋白基因的表达可能是胚胎神经系统发育以及胚胎后大脑中干细胞和增殖前体的标志。
Background: The intermediate filament Nestin has been reported as a marker for stem cells and specific precursor cell populations in the developing mammalian central nervous system (CNS). Nestin expressing precursors may give rise to neurons and glia. Mouse nestin expression starts at the onset of neurulation in the neuroectodermal cells and is dramatically down regulated when progenitor cells differentiate and become postmitotic. It has been reported that in the adult zebrafish ( Danio rerio) active neurogenesis continues in all major subdivisions of the CNS, however few markers for zebrafish precursors cells are known, and Nestin has not been described in zebrafish.Results: We cloned a zebrafish nestin gDNA fragment in order to find a marker for precursor cells in the developing and postembryonic brain. Phylogenetic tree analysis reveals that this zebrafish ortholog clusters with Nestin sequences from other vertebrates but not with other intermediate filament proteins. We analyzed nestin expression from gastrula stage to 4 day larvae, and in postembryonic brains. We found broad expression in the neuroectoderm during somitogenesis. In the larvae, nestin expression progressively becomes restricted to all previously described proliferative zones of the developing and postembryonic central nervous system. nestin expressing cells of the forebrain also express PCNA during late embryogenesis, identifying them as proliferating precursor or neural stem cells. nestin is also expressed in the cranial ganglia, in mesodermal precursors of muscle cells, and in cranial mesenchymal tissue.Conclusion: Our data demonstrate that in zebrafish, like in mammals, the expression of the intermediated neurofilament nestin gene may serve as a marker for stem cells and proliferating precursors in the developing embryonic nervous system as well as in the postembryonic brain.