Chromatin insulators specifically associate with different levels of higher-order chromatin organization in Drosophila.
Chromatin insulators specifically associate with different levels of higher-order chromatin organization in Drosophila.
复制标题
染色质绝缘体与果蝇中不同水平的高阶染色质组织特异性相关。
DOI：
10.1007/s00412-009-0246-0
复制
发表时间：
2010
期刊：
影响因子：
1.6
通讯作者：
Labrador,Mariano
中科院分区：
文献类型：
--
作者：
Wallace,HeatherA;Plata,MariaP;Kang,Hyuck-Joon;Ross,Misty;Labrador,Mariano
染色质绝缘体对于后生动物中基因的正确时空表达是必需的。在这里，我们分析了果蝇多线染色体 2R 染色体 56F-58A 区域上绝缘体蛋白的分布，以评估染色质绝缘体在塑造基因组结构中的作用。数据显示，毛翼蛋白 [Su(Hw)] 的抑制子存在于与绝缘体蛋白差异相关的三种结构中：带、带间和共表达基因的多基因域。结果表明，条带通常由属于含有一个或多个 Su(Hw) 结合位点的基因的染色质浓缩形成，而在条带间，Su(Hw) 位点似乎与开放染色质相关。此外，该区域中的共表达基因簇形成以缺乏 CP190 和 BEAF-32 绝缘体蛋白为特征的条带。这种模式与特定染色质标记的分布相关，并且在护理细胞中保守，表明这种组织可能不限于一种细胞类型，而是代表间期染色体的基本组织。
Chromatin insulators are required for proper temporal and spatial expression of genes in metazoans. Here, we have analyzed the distribution of insulator proteins on the 56F–58A region of chromosome 2R inDrosophilapolytene chromosomes to assess the role of chromatin insulators in shaping genome architecture. Data show that the suppressor of Hairy-wing protein [Su(Hw)] is found in three structures differentially associated with insulator proteins: bands, interbands, and multi-gene domains of coexpressed genes. Results show that bands are generally formed by condensation of chromatin that belongs to genes containing one or more Su(Hw) binding sites, whereas, in interbands, Su(Hw) sites appear associated with open chromatin. In addition, clusters of coexpressed genes in this region form bands characterized by the lack of CP190 and BEAF-32 insulator proteins. This pattern correlates with the distribution of specific chromatin marks and is conserved in nurse cells, suggesting that this organization may not be limited to one cell type but represents the basic organization of interphasic chromosomes.