Bio-inspired synthesis of PEGylated Polypyrrole@Polydopamine Nanocomposite as Theranostic agent for T1-Weighted MR imaging guided Photothermal Therapy
Bio-inspired synthesis of PEGylated Polypyrrole@Polydopamine Nanocomposite as Theranostic agent for T1-Weighted MR imaging guided Photothermal Therapy
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仿生合成聚乙二醇化聚吡咯@聚多巴胺纳米复合材料作为T-1加权磁共振成像引导光热治疗的治疗诊断剂
DOI：
10.1039/c6tb02740g
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发表时间：
2017
影响因子：
7
通讯作者：
Haibo Xu
中科院分区：
文献类型：
--
作者：
Zhe Yang;Jinghua Ren;Zhilan Ye;Wei Zhu;Liji Xiao;Li Zhang;Qianyuan He;Zushun Xu;Haibo Xu
聚吡咯纳米粒子（PPy）为基础的磁共振成像（MRI）引导光热治疗（PTT）的治疗诊断剂，近年来受到越来越多的关注。然而，成本和生物相容性的限制仍然为我们提供了改进这些药物的机会。考虑到聚多巴胺（PDA）的多功能性，设计并制备了PEG化PPy@Fe3+螯合PDA纳米复合材料（PPDE）。通过调节多巴胺和PPys的比例，可以得到具有均匀核壳结构的PPDE。在这种纳米复合物中，壳赋予纳米颗粒良好的生物相容性和MRI信号增强能力。此外，PPy核在肿瘤的光热消融中起作用。与纯PDA纳米粒子相比，PPDE具有更高的近红外吸收和更好的光热性能，这得益于核的高光热转换。此外，所获得的PPDE提供了显着的MRI信号增强在体外和体内成像与高纵向弛豫率（r1 = 5.055 mM-1 s-1）。静脉注射后，PPDE表现出有效的肿瘤蓄积，如MRI所示，并通过生物分布分析验证。在NIR照射下，PPDE显示出对4 T1细胞的高效光热消融。值得注意的是，通过相关的MTT试验和组织学分析证实了PPDE具有优异的生物相容性。这项工作实现了一个例子，利用PPy和PDA的固有优势，在一个单一的单位，并探索其潜力的T1 MRI引导PTT。
Polypyrrole nanoparticle (PPy) based theranostic agents for magnetic resonance imaging (MRI) guided photothermal therapy (PTT) have received increasing attention in recent years. However, the limitations of cost and biocompability still offer us opportunities to improve these agents. Considering the versatile character of polydopamine (PDA), PEGylated PPy@Fe3+-chelated PDA nanocomposites (PPDEs) were designed and prepared in an easy way. PPDE with a uniform core–shell structure could be obtained by adjusting the ratio of dopamine and PPys. In this nanocomplex, the shells confer the nanoparticles with good biocompability and MRI signal enhancing ability. Moreover, the PPy cores play a role in photothermal ablation of tumors. Compared with pure PDA nanoparticles, the PPDEs have higher NIR absorbance and better photothermal capability benefitting from the high photothermal conversion of the cores. Additionally, the obtained PPDEs provide significant MRI signal enhancement for both in vitro and in vivo imaging with high longitudinal relaxivity (r1 = 5.055 mM−1 s−1). After intravenous injection, the PPDEs exhibited valid tumor accumulation, as revealed by MRI and verified by biodistribution analysis. Under NIR irradiation, the PPDEs showed highly effective photothermal ablation of 4T1 cells. Notably, excellent biocompability of the PPDEs was confirmed by a relevant MTT assay and histologic analysis. This work achieved an example of exploiting the inherent advantages of PPy and PDA within a single unit and exploring its potential for T1 MRI-guided PTT.