Spatiotemporal immune zonation of the human kidney
Spatiotemporal immune zonation of the human kidney
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DOI：
10.1126/science.aat5031
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发表时间：
2019-09-27
期刊：
影响因子：
56.9
通讯作者：
Clatworthy, Menna R.
中科院分区：
文献类型：
--
作者：
Stewart, Benjamin J.;Ferdinand, John R.;Clatworthy, Menna R.
组织驻留免疫细胞对于器官稳态和防御是重要的。上皮细胞可能直接或通过与免疫细胞的相互作用而促进这些功能。我们使用单细胞RNA测序来解析人类肾脏的时空免疫拓扑结构。我们揭示了解剖学定义的免疫基因的表达模式内的上皮隔室，抗菌肽转录明显的盆腔上皮中的成熟。但不是胎儿肾在胎儿和成熟肾脏中，组织驻留的髓样和淋巴样免疫细胞网络是明显的，出生后获得的转录程序，促进感染防御能力。上皮免疫串扰精心安排抗菌巨噬细胞和中性粒细胞定位于最容易感染的肾脏区域。总的来说，我们的研究提供了一个全球性的概述，人类肾脏的免疫景观是如何分区的，以应对占主导地位的免疫挑战。
Tissue-resident immune cells are important for organ homeostasis and defense. The epithelium may contribute to these functions directly or by cross-talk with immune cells. We used single-cell RNA sequencing to resolve the spatiotemporal immune topology of the human kidney. We reveal anatomically defined expression patterns of immune genes within the epithelial compartment, with antimicrobial peptide transcripts evident in pelvic epithelium in the mature. but not fetal, kidney. A network of tissue-resident myeloid and lymphoid immune cells was evident in both fetal and mature kidney, with postnatal acquisition of transcriptional programs that promote infection-defense capabilities. Epithelial-immune cross-talk orchestrated localization of antibacterial macrophages and neutrophils to the regions of the kidney most susceptible to infection. Overall, our study provides a global overview of how the immune landscape of the human kidney is zonated to counter the dominant immunological challenge.