Trans-species activation of human T cells by rhesus macaque CD1b molecules
Trans-species activation of human T cells by rhesus macaque CD1b molecules
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DOI：
10.1016/j.bbrc.2008.10.075
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发表时间：
2008-12-19
影响因子：
3.1
通讯作者：
Sugita, Masahiko
中科院分区：
文献类型：
--
作者：
Morita, Daisuke;Katoh, Kumiko;Sugita, Masahiko
尽管非人类灵长类动物作为人类传染病的模型至关重要，但在这些物种中，第一组CD1基因和蛋白的特征一直很差。在这里，我们从猕猴淋巴结中分离出CD1a、CD1B和CD1C cDNA，编码全长CD1蛋白，该蛋白可被抗人CD1a的单抗识别。CD1b和CD1c分子。猴1组CD1亚型含有氨基酸残基和基序，与人类1组CD1分子一样，对分子内二硫键的形成、N-连接糖基化和内体转运至关重要。值得注意的是，猴子CD1b分子能够将分枝杆菌糖脂呈递给人类CM限制的T细胞，为其抗原呈递功能提供了直接证据。这也是第一次检测到由I组CD1分子介导的跨物种交叉反应。综上所述，这些结果强调了人类和恒河猴之间第一组CD1系统的实质性保护。(C)2008 Elsevier Inc.保留所有权利。
Despite crucial importance of non-human primates as a model of human infectious diseases, group 1 CD1 genes and proteins have been poorly characterized in these species. Here, we isolated CD1A CD1B, and CD1C cDNAs from rhesus macaque lymph nodes that encoded full-length CD1 proteins recognized specifically by monoclonal antibodies to human CD1a. CD1b, and CD1c molecules, respectively. The monkey group 1 CD1 isoforms contained amino acid residues and motifs known to be critical for intramolecular disulfide bond formation, N-linked glycosylation, and endosomal trafficking as in human group 1 CD1 molecules. Notably, monkey CD1b molecules were capable of presenting a mycobacterial glycolipid to human CM-restricted T cells, providing direct evidence for their antigen presentation function. This also detects for the first time a trans-species crossreaction mediated by group I CD1 molecules. Taken together, these results underscore substantial conservation of the group 1 CD1 system between humans and rhesus macaque monkeys. (C) 2008 Elsevier Inc. All rights reserved.