Genetic and antigenic characterization of complete genomes of Type 1 Porcine Reproductive and Respiratory Syndrome viruses (PRRSV) isolated in Denmark over a period of 10 years
Genetic and antigenic characterization of complete genomes of Type 1 Porcine Reproductive and Respiratory Syndrome viruses (PRRSV) isolated in Denmark over a period of 10 years
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DOI：
10.1016/j.virusres.2013.10.009
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发表时间：
2013-12-26
期刊：
影响因子：
5
通讯作者：
Larsen, Lars E.
中科院分区：
文献类型：
--
作者：
Kvisgaard, Lise K.;Hjulsager, Charlotte K.;Larsen, Lars E.
由猪繁殖与呼吸综合征（PRRS）病毒引起的猪繁殖与呼吸综合征（PRRS）被认为是世界上最具破坏性的猪疾病之一。PRRS病毒分为两种主要基因型，1型和2型，基因型之间和基因型内部具有明显的多样性。在丹麦，50%以上的畜群感染了1型和/或2型PRRSV。本研究的主要目的是检查1型病毒在有限引进新动物和精液的种群中的遗传多样性和漂移。从2003年至2013年2月采集的病毒中共获得43个ORF5和42个ORF7核苷酸序列。ORF5核苷酸序列的系统发育分析表明，丹麦分离株在1亚型内形成两个主要聚类。ORF5与Lelystad病毒1亚型（LV）的核苷酸同源性为84.9 ~ 98.8%，ORF7为90.7 ~ 100%。在丹麦病毒中，ORF5和ORF7的成对核苷酸同源性分别为81.2-100%和88.9-100%。8株丹麦PRRSV 1型病毒的全基因（包括5′端和3′端核苷酸）测序结果显示，基因组长度在14,876 ~ 15,098个核苷酸之间，丹麦病毒之间的配对核苷酸同源性为86.5 ~ 97.3%，与LV的同源性为88.7 ~ 97.9%。该研究强烈表明，在丹麦至少有两次1型PRRSV的独立传入，对全基因组的分析显示该病毒在几个区域有明显的漂移。(C) 2013年作者。Elsevier B.V.版权所有。
Porcine Reproductive and Respiratory Syndrome (PRRS) caused by the PRRS virus (PRRSV) is considered one of the most devastating swine diseases worldwide. PRRS viruses are divided into two major genotypes, Type 1 and Type 2, with pronounced diversity between and within the genotypes. In Denmark more than 50% of the herds are infected with Type 1 and/or Type 2 PRRSV. The main objective of this study was to examine the genetic diversity and drift of Type 1 viruses in a population with limited introduction of new animals and semen. A total of 43 ORF5 and 42 ORF7 nucleotide sequences were obtained from viruses collected from 2003 to February 2013. Phylogenetic analysis of ORF5 nucleotide sequences showed that the Danish isolates formed two major clusters within the subtype 1. The nucleotide identity to the subtype 1 protogenotype Lelystad virus (LV) spanned 84.9-98.8% for ORF5 and 90.7-100% for ORF7. Among the Danish viruses the pairwise nucleotide identities in ORF5 and ORF7 were 81.2-100% and 88.9-100%, respectively. Sequencing of the complete genornes, including the 5'- and 3'-end nucleotides, of 8 Danish PRRSV Type 1 showed that the genome lengths differed from 14,876 to 15,098 nucleotides and the pairwise nucleotide identity among the Danish viruses was 86.5-97.3% and the identity to LV was 88.7-97.9%. The study strongly indicated that there have been at least two independent introductions of Type 1 PRRSV in Denmark and analysis of the full genomes revealed a significant drift in several regions of the virus. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.