QTL mapping for low-dose ethanol activation in the LXS recombinant inbred strains
QTL mapping for low-dose ethanol activation in the LXS recombinant inbred strains
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DOI：
10.1111/j.1530-0277.2006.00137.x
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发表时间：
2006-07-01
影响因子：
3.2
通讯作者：
Johnson, TE
中科院分区：
文献类型：
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作者：
Downing, C;Carosone-Link, P;Johnson, TE
背景资料：利用C57 BL/6 J（B6）和DBA/2 J（D2）小鼠群体定位了大多数小鼠行为性状的数量性状基因座（QTL）。在来自其他祖先的群体中鉴定行为的QTL也很重要。我们报告的结果QTL定位低剂量（乙醇）自发激活（LDA）使用最近开发的LXS重组近交系（RI），来自近交长睡眠（ILS）和近交短睡眠（ISS）的祖先。LXS RI面板具有额外的遗传变异，和更大的权力，由于大量的菌株，与其他RI面板和菌株cross.Methods：小鼠进行了测试，使用3天的协议，其中活动水平进行了监测，每天15分钟。在第1天，记录基线活动;在第2天，在测试前给小鼠注射生理盐水;在第3天，给小鼠注射1.8 g/kg乙醇并进行测试。结果：在第2、3、4、7、8、12和13号染色体上发现了几个提示性QTL;其中3个QTL是性别特异的。在第4号和第8号染色体上发现了两个明显新颖的LDA QTL。其他QTL似乎复制先前鉴定的LDA QTL。这些重复的QTL将在随后的研究中进行，旨在确定候选基因。
Background: Most mouse quantitative trait loci (QTLs) for behavioral traits have been mapped using populations of mice derived from C57BL/6J (B6) and DBA/2J (D2). It is also important to identify QTLs for behavior in populations derived from other progenitors. We report results from QTL mapping for low-dose (ethanol) locomotor activation (LDA) using the recently developed LXS recombinant inbred (RI) strains, derived from Inbred Long Sleep (ILS) and Inbred Short Sleep (ISS) progenitors. The LXS RI panel has additional genetic variation, and greater power due to a larger number of strains, compared with other RI panels and strain crosses.Methods: Mice were tested using a 3-day protocol in which activity levels were monitored for 15 minutes each day. On day 1, baseline activity was recorded; on day 2, mice were injected with saline before testing; and on day 3, mice were injected with 1.8 g/kg ethanol and tested.Results: Several suggestive QTLs were found, on chromosomes 2, 3, 4, 7, 8, 12, and 13; 3 of these QTLs were sex-specific.Conclusions: Two apparently novel LDA QTLs were identified, on chromosomes 4 and 8. The other QTLs appear to replicate previously identified LDA QTLs. These replicated QTLs will be pursued in subsequent studies designed to identify candidate genes.