The protein Hrb57A of Drosophila melanogaster closely related to hnRNP K from vertebrates is present at sites active in transcription and coprecipitates with four RNA-binding proteins
The protein Hrb57A of Drosophila melanogaster closely related to hnRNP K from vertebrates is present at sites active in transcription and coprecipitates with four RNA-binding proteins
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DOI：
10.1016/s0378-1119(00)00027-5
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发表时间：
2000-03-07
期刊：
影响因子：
3.5
通讯作者：
Saumweber, H
中科院分区：
文献类型：
--
作者：
Hovemann, BT;Reim, I;Saumweber, H
hnRNP K蛋白是主要的hnRNA结合蛋白之一，具有对富含胞苷序列的强烈偏好。我们克隆了一个与这种脊椎动物蛋白质密切相关的果蝇hnRNP蛋白质，该蛋白质首先由单克隆抗体Q18鉴定，由位于多线染色体57 A的基因编码，因此被命名为Hrb 57 A。Hrb 57 A KH结构域的氨基酸序列及其在蛋白质中的整体组织与脊椎动物蛋白质非常相似。作为脊椎动物中的hnRNP K，M-r 55 000果蝇Hrb 57 A/Q18蛋白在体外与poly（C）强烈结合，并且普遍存在于具有转录活性的细胞核中。在多线染色体上，它存在于许多团簇和小团簇中。Hrb 57 A/Q18特异性共沉淀四种其它蛋白质：Hrb 87 F/P11 a果蝇hnRNP A1同源物、hnRNA结合蛋白S5、含有RNA识别基序的蛋白NonA和含有RNA结合锌指的蛋白质，其在蜕皮激素喷雾PEP/X4上，（C）2000 Elsevier Science B. V.保留所有权利。
The hnRNP K protein is among the major hnRNA-binding proteins with a Strong preference for cytidine-rich sequences. We have cloned a Drosophila hnRNP protein closely related to this vertebrate protein, The protein first identified by the monoclonal antibody Q18 is encoded by a gene located in 57A on polytene chromosomes and has been consequently named Hrb57A. The amino acid sequence of the Hrb57A KH domains and their overall organisation in the protein are remarkably similar to the vertebrate proteins. As the hnRNP K in vertebrates the M-r 55 000 Drosophila Hrb57A/Q18 protein strongly binds to poly(C) in vitro and is ubiquitously present in nuclei active in transcription. On polytene chromosomes it is found in many puffs and minipuffs. Hrb57A/Q18 specifically coprecipitates four other proteins: Hrb87F/P11 a Drosophila hnRNP Al homologue, the hnRNA-binding protein S5, the RNA recognition motif-containing protein NonA and the RNA-binding zinc finger-containing protein on ecdysone puffs PEP/X4, (C) 2000 Elsevier Science B.V. All rights reserved.